Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 19,669 results for: MUT

Clear keywords
Showing: 1781 - 1800 of 19669 results

  1. G protein alpha-s(alpha3beta5/G226A)

    Plasmid
    #55798
    Purpose
    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A in alpha…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55798-plasmid-map-sequence-id-112177

    Enlarge

  4. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid
    #55797
    Purpose
    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55797-plasmid-map-sequence-id-112181

    Enlarge

  7. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

    Enlarge

  9. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58492-plasmid-map-sequence-id-90361

    Enlarge

  10. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Article
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

    Enlarge

  11. ST-Tstem(5A)-GFP

    Plasmid
    #162502
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The mutated Tac stem region is inserted within ST…
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162502-plasmid-map-sequence-id-323507

    Enlarge

  12. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV1)

    Viral Prep
    #26969-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV5)

    Viral Prep
    #26969-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV/D377Y-mPCSK9 (AAV8)

    Viral Prep
    #58376-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV/D377Y-mPCSK9 (#58376). In addition to the viral particles, you will also receive purified pAAV/D377Y-mPCSK9 plasmid DNA. hAAT-driven expression of mutant (D377Y) murine PCSK9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jacob Bentzon
    Article
    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do
    Promoter
    HCRApoE/hAAT
    Available Since
    July 30, 2025
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV9)

    Viral Prep
    #26969-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)

    Viral Prep
    #20298-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV2)

    Viral Prep
    #26969-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. AAV-EF1a-BbTagBY (AAV9)

    Viral Prep
    #45185-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Dawen Cai, Joshua Sanes
    Article
    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi
    Promoter
    EF1a
    Tags
    TagBFP, EYFP, or none.
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV/D374Y-hPCSK9 (AAV8)

    Viral Prep
    #58379-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV/D374Y-hPCSK9 (#58379). In addition to the viral particles, you will also receive purified pAAV/D374Y-hPCSK9 plasmid DNA. hAAT-driven expression of mutant (D374Y) human PCSK9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jacob Bentzon
    Article
    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do
    Promoter
    HCRApoE/hAAT
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only

  20. AAV-FLEX-rev-ChR2(H134R)-mCherry (AAV1)

    Viral Prep
    #18916-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-FLEX-rev-ChR2(H134R)-mCherry (#18916). In addition to the viral particles, you will also receive purified AAV-FLEX-rev-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, cre-dependent, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Scott Sternson
    Article
    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.
    Promoter
    CAG
    Tags
    mCherry (Cre-dependent)
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1781 - 1800 of 19669 results