We narrowed to 5,335 results for: cycs

1. pUASTattb-cAMPr Y205A, R626A

   Plasmid

   #84932

   Purpose

   cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

   Depositor

   Justin Blau

   Article

   Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

   Insert

   cAMPr Y205A R626A
   

   Use

   Tags

   Expression

   Insect

   Mutation

   PKA-C Y205A, PKA-R R626A

   Promoter

   5X UAS

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pABM-D4-miR30-CypA

   Plasmid

   #132934

   Purpose

   Lentivector for CypA knockdown and blasticidin resistance

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   CypA shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR47

   Plasmid

   #69258

   Purpose

   pmyo-3::CRE for integration on ttTi14024, Chr X

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   myo-3

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HGXPRT

   Plasmid

   #64201

   Purpose

   Expression of Plasmodium falciparum HGXPRT

   Depositor

   Vern Schramm

   Article

   Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

   Insert

   Hypoxanthine guanine xanthine phosphoribosyltransferase
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. SEPT5 Mut(S161A/S327A)

   Plasmid

   #27275

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

   Insert

   Septin 5 (SEPTIN5 Human)
   

   Use

   Tags

   Myc (MASMQKLISEEDL)

   Expression

   Mammalian

   Mutation

   S161A and S327A

   Promoter

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-N1-mSNX27 RNAi-resistant

   Plasmid

   #163620

   Purpose

   mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   mouse SNX27 RNAi-resistant (Snx27 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   RNAi resistance

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPU-Empty-miR30-L1221

   Plasmid

   #132940

   Purpose

   All-in-one control rescue-control shRNA knockdown vector

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPU-huTRIMmiR30-L1221

   Plasmid

   #132937

   Purpose

   All-in-one huTRIM5 rescue-control shRNA knockdown

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   human TRIM5
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HisSumoOphP

   Plasmid

   #189672

   Purpose

   HisSUMO tagged OphP to express OphP in pichia pastorias.

   Depositor

   James Naismith, Markus Künzler

   Article

   Emmanuel et al bioRxiv 2022.07.21.500988

   Insert

   HisSumoOphP
   

   Use

   Tags

   HisSumo tagged

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-MKRN1 H307E

    Plasmid

    #78749

    Purpose

    To overexpress MKRN1 H307E in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H307E

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHJ18

    Plasmid

    #48357

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB303-Tier1-PhCMV-TetR_VPR

    Plasmid

    #169575

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-dependent VPR-based transactiavtor (PhCMV-TetR-VPR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB301-Tier1-PhCMV-rTetR_VPR

    Plasmid

    #169579

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-activated VPR-based transactiavtor (PhCMV-rTetR-VPR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    reverse tetracycline-controlled transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ292-D570A

    Plasmid

    #129679

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D570A; AaCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. SEPT5 Mut(S327A)

    Plasmid

    #27274

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

    Insert

    Septin 5 (SEPTIN5 Human)
    

    Use

    Tags

    Myc (MASMQKLISEEDL)

    Expression

    Mammalian

    Mutation

    S327A

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pHJ41

    Plasmid

    #48360

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-FLAG/MKRN1(1-369)

    Plasmid

    #78753

    Purpose

    To overexpress MKRN1(1-369) in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    1-369

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR35

    Plasmid

    #69256

    Purpose

    phsp16.41::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.41

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUPI

    Plasmid

    #80279

    Purpose

    A bicistronic lentiviral vector used to stably express cDNAs.

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    Puro resistance cassette, IRES, cloning site
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pADH118-2

    Plasmid

    #90984

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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