We narrowed to 7,267 results for: trac

1. pPy-CAG-Cre::ERT2-IRES-BSD

   Plasmid

   #48760

   Purpose

   CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Insert

   Cre::ERT2
   

   Use

   Ppy

   Tags

   ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-PLIN2-tdTOMATO

   Plasmid

   #172533

   Purpose

   PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F, and R415H mutations in …

   Promoter

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 5HT2A_NB2_1

   Plasmid

   #127690

   Purpose

   Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   5HT2A_NB2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRY2low-mCherry

   Plasmid

   #104065

   Purpose

   Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2low-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2 aa 1-491 truncation, R489E A491D

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Use

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTREX GPR68_NB2_2

   Plasmid

   #127691

   Purpose

   Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   GPR68_NB2_2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBpuro-COX2-EBFP2

   Plasmid

   #184720

   Purpose

   Exogenous COX2

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   COX2-EBFP2 (PTGS2 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

   Plasmid

   #171432

   Purpose

   Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-hH3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2365-Tier1-PhCMV-TetR

   Plasmid

   #169566

   Purpose

   Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven tetracycline repressor protein class B from transposon Tn10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid

    #212120

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    

    Use

    Tags

    His6 and SUMO-TAG35

    Expression

    Bacterial

    Mutation

    E35TAG (within SUMO encoding sequence)

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAP24

    Plasmid

    #70190

    Purpose

    Modular vector for secreted luciferase reporter expression

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

    Insert

    sLucopt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid

    #133317

    Purpose

    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus thermophilus #3)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    xylose

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Fast-GCaMP-EF20

    Plasmid

    #52645

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF20
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D362N/D366N mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

14. FKBP-AP180-GFP

    Plasmid

    #186577

    Purpose

    Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP180 (Snap91 Rat)
    

    Use

    Tags

    FKBP N-terminal GFP C-terminal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Histone H2B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45216

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    Histone H2B-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP Tensin2

    Plasmid

    #105298

    Purpose

    To study localization and function of Tensin2

    Depositor

    David Critchley, Kenneth Yamada

    Article

    Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

    Insert

    Tensin2 (TNS2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    lacks N-terminal C1 domain

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

    Plasmid

    #171427

    Purpose

    Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-CMV-NLS-dCas9-NLS

    Plasmid

    #99908

    Purpose

    For the expression of NLS-dCas9-NLS

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-dCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEINO3

    Plasmid

    #179487

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    TetR/A
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid

    #74709

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only