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pSW455 - pCLV3

Plasmid

#115979

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta1

Plasmid

#101289

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

1-375 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCoofy48

Plasmid

#55186

Purpose

Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pVL26 - pSCR

Plasmid

#116021

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pSCR

Use

Unspecified

Mutation

T171A by SDM to abolish Eco31I site

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMTIKA

Plasmid

#22061

Depositor

Maria Castro

Article

Xiong et al J Virol. 2006 Jan . 80(1):27-37.

Insert

mCMV-TetOn expression cassette

Expression

Mammalian

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
TC513

Plasmid

#122616

Purpose

Punc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherry

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

SV40 NLS::rtetR::Zip(+)::SL2::mCherry

Expression

Worm

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSW614 - pREV

Plasmid

#115989

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pREV

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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BcLOV-mCh-EGFR

Plasmid

#208274

Purpose

Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCherry-EGFR

Use

Lentiviral

Tags

mCherry

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPD1029 ZF43x6-Compact(CMVMin)_EYFP

Plasmid

#138913

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(CMVmin)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact(CMVmin)

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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EB3-3xmTq2-N1

Plasmid

#112929

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

3x mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSW618 - pAHP6

Plasmid

#115991

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pAHP6

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSW615 - pATHB-8

Plasmid

#115990

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pATHB-8

Use

Unspecified

Mutation

A1287T to abolish Eco31I site

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVL15 - tSCR

Plasmid

#116030

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tSCR

Use

Unspecified

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Tags

6X-His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shTFRC

Plasmid

#169882

Purpose

Suppress TRFC

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

shRNA against TFRC

Use

Lentiviral and RNAi

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-104

Plasmid

#79842

Purpose

Expresses NLS-PpsR2-VP16 under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-VP16

Tags

Nuclear localization signal of nuclear cap-bindin…

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pT3TS-Cre

Plasmid

#47117

Purpose

Can be used to in vitro transcribe Cre recombinase.

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

Cre

Promoter

Lac

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GI-ZFP2

Plasmid

#42215

Purpose

Expresses GI-ZFP2 for light-inducible activation of gene expression

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

GIGANTEA-FLAG-ZFP2 (GI Mustard Weed, Synthetic)

Tags

FLAG

Promoter

CMV

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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3xmTurquoise2_UtrCH

Plasmid

#112963

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

UtrCH

Tags

3x mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHRW-CRY2olig-VHH(GFP)

Plasmid

#126023

Purpose

express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHRolig-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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