165,173 results

1. mKeima-Red-N1

   Plasmid

   #54597

   Purpose

   Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mKeima-Red

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-HA-UBE3A-IRES-Puro

   Plasmid

   #107349

   Purpose

   Expresses HA tagged UBE3A in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3A (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. FLAG-NLRC5

   Plasmid

   #37521

   Depositor

   Thomas Kufer

   Article

   Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

   Insert

   NLRC5 (NLRC5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (AAV1)

   Viral Prep

   #172922-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (#172922). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 plasmid DNA. Syn-driven expression of calcium sensor jGCaMP8m in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Promoter

   Syn

   Tags

   mRuby3

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

5. pHR' CMV GFP

   Plasmid

   #14858

   Depositor

   Inder Verma

   Article

   Miyoshi et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

   Insert

   CMV-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b(+)_EGFP_NbALFA

   Plasmid

   #136626

   Purpose

   Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCarmine in pRSETB

   Plasmid

   #109484

   Purpose

   bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

   Depositor

   Oliver Griesbeck

   Article

   Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

   Insert

   mCarmine
   

   Use

   Tags

   N-terminal histidine tag in vector

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. RED

   Plasmid

   #139835

   Purpose

   Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9 protein contains two mutations: D10A and H8…

   Promoter

   tet-inducible CMV

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol-Promoter:NLS-tdMCP-eGFP

   Plasmid

   #86248

   Purpose

   For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

   Depositor

   Florence Marlow

   Article

   Campbell et al Development. 2015 Apr 1;142(7):1368-74.

   Insert

   beta Actin promoter:NLS-tdMCPeGFP
   

   Use

   Used with transposase to make transient or stable…

   Tags

   NLS-tdMCP-eGFP

   Expression

   Mutation

   Promoter

   Beta Actin

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPL

    Plasmid

    #110743

    Purpose

    Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin and p10 promoters

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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