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We narrowed to 15,064 results for: pET

Showing: 4501 - 4550 of 15064 results
  1. 371-GFP

    Plasmid
    #97030
    Purpose
    His6-GFP-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    GFP fusion, His6 tag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. 371-Bc

    Plasmid
    #97028
    Purpose
    yORF-tev-His6
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pMH_MBP_AtCCA1_88-198

    Plasmid
    #238517
    Purpose
    Bacterial expression of A. thaliana CCA1 88-198
    Depositor
    Insert
    AtCCA1 (CCA1 Mustard Weed)
    Use
    Tags
    2x Strep, 6x His, MBP, N-term, TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. 371-T

    Plasmid
    #97035
    Purpose
    His6-Thioredoxin-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 tag, TEV cleavage site, and Thioredoxin funs…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. 371-R

    Plasmid
    #97033
    Purpose
    StrepII-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    StrepII tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. 371-S

    Plasmid
    #97034
    Purpose
    His6-Sumo-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 tag, SUMO domain, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. 371-O

    Plasmid
    #97032
    Purpose
    His6-Mocr-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 tag, TEV cleavage site, and mOCR fusion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. 371-L

    Plasmid
    #97031
    Purpose
    FLAG-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    FLAG tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201819
    Purpose
    A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter
    Depositor
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pOTTC1751
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pCJ199

    Plasmid
    #162672
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Insert
    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Insert
    MSP1D1Q1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  15. pMH_MBP_AtCK2beta1_95-287

    Plasmid
    #238519
    Purpose
    Bacterial expression of A. thaliana CK2beta1 95-287
    Depositor
    Insert
    AtCK2beta1 (CKB1 Mustard Weed)
    Use
    Tags
    2x Strep, 6x His, MBP, N-term, TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. CAPN2A-c002

    Plasmid
    #205104
    Purpose
    CAPN2 protein expression
    Depositor
    Insert
    CAPN2 (CAPN2 Human)
    Use
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  17. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Promoter
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  18. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Promoter
    Available Since
    Nov. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. KSP_HNL

    Plasmid
    #129772
    Purpose
    Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays
    Depositor
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367
    Promoter
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  20. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  21. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Use
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  22. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  23. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Insert
    ppie (PPIE Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Promoter
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  24. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  25. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  26. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  27. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  28. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  29. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  30. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  31. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. pCJ205

    Plasmid
    #162676
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224A, C529A; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  33. pCJ198

    Plasmid
    #162671
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    F495I; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  34. pCJ196

    Plasmid
    #162669
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S225A; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  35. pCJ204

    Plasmid
    #162677
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224H, C529F; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  36. pCJ201

    Plasmid
    #162674
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.
    Depositor
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224W, C529SS; codon optimized for expression in …
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    Insert
    hypothetical acetic acid resistance protein B
    Use
    Tags
    NusA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  38. pMulti_K_ccdB

    Plasmid
    #223852
    Purpose
    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)
    Depositor
    Type
    Empty backbone
    Use
    Golden-gate acceptor
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  39. pMulti_A_ccdB

    Plasmid
    #223851
    Purpose
    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)
    Depositor
    Type
    Empty backbone
    Use
    Golden-gate acceptor
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  40. Ubiquiton In Vitro Kit

    Plasmid Kit
    #1000000242
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Available Since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  41. 13SA-csy4

    Plasmid
    #97037
    Purpose
    Spectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectors
    Depositor
    Insert
    Cys4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  42. BAZ2A

    Plasmid
    #73261
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    BAZ2A (BAZ2A Human)
    Use
    Tags
    His-6-SUMO
    Expression
    Bacterial
    Mutation
    pfam00628:PHD 1652-1696
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  43. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  44. Rab11A[8-175]-His-C

    Plasmid
    #228040
    Purpose
    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling.
    Depositor
    Insert
    Rab11A[8-175]-His-C (RAB11A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4501 - 4550 of 15064 results