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PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Promoter

SP6

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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FL-BCAN-lentiviral-GFP

Plasmid

#29704

Depositor

Mariano Viapiano

Publication

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

Brevican (BCAN Human)

Use

Lentiviral

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
ATF4 7: 5’3’ATF4.GFP

Plasmid

#21854

Depositor

David Ron

Publication

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Vector derived viral termination and poly-adenyla…

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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M3927 ura3::KanMX3 Disruptor Converter

Plasmid

#51682

Purpose

Yeast marker swap plasmid for converting URA3 to KanMX3

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)(FRT)MCS

Plasmid

#31377

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(loxP)MCS

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LCMV:GFP(FRT)DsRed

Plasmid

#31378

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)DsRed

Use

Flp/frt

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

J23107

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FKBP_LD 0_TEV

Plasmid

#58877

Purpose

MESA protease chain with Rapamycin FKBP ectodomain and no linker domain

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pICSL50009

Plasmid

#50309

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pICH44022

Plasmid

#50330

Depositor

Sylvestre Marillonnet

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CDS, P19 suppressor of gene silencing (Tomato Bushy Stunt Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EcMBP165-cpGFP.PPYF.T203V.pRSET

Plasmid

#33373

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Publication

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

Improved MBP based Maltose Biosensor

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B

Plasmid

#21683

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

maternal expression at 31B (me31B Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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px330 SUZ12 - pDY083

Plasmid

#171109

Purpose

Expresses Cas9 and an sgRNA targeting SUZ12

Depositor

Thomas Cech

Publication

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2 TetO(fl)-2

Plasmid

#24416

Depositor

Edward Hsiao

Publication

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Gateway Cloning

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Plasmid

#24418

Depositor

Edward Hsiao

Publication

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Use

Mouse Targeting

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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3539 pMIG Bax

Plasmid

#8788

Depositor

Stanley Korsmeyer

Publication

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bax (Bax Mouse)

Use

Retroviral

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLV-mCherry-Stim1

Plasmid

#186620

Purpose

Third generation lentiviral vector expressing Stim1-wt fused to mCherry

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Publication

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKL2310

Plasmid

#187875

Purpose

Empty pVS1-based INTEGRATE vector with loxP cargo

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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