We narrowed to 2,475 results for: T2A

1. pAAV hSynapsin SV-tag

   Plasmid

   #163685

   Purpose

   express SV-Tag construct in a Cre-independent manner

   Depositor

   Bernardo Sabatini

   Article

   Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

   Insert

   synatophysin 9X HA tag T2A tdtomato
   

   Use

   AAV

   Tags

   9X HA

   Expression

   Mammalian

   Promoter

   synapsin

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEntry Tshld(FKBP F36V)-GFP

   Plasmid

   #53260

   Purpose

   pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

   Depositor

   Matthew Pratt

   Article

   Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

   Insert

   Tshld(FKBP F36V)-GFP
   

   Tags

   EGFP, Flag, and HA

   Mutation

   FKBP F36V

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQP-AR10

   Plasmid

   #138980

   Purpose

   Endogenous RAS regulation using light-controlled intrabody

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
   

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SEPLuc

   Plasmid

   #141102

   Purpose

   bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEP-Nanoluc
   

   Use

   Lentiviral

   Promoter

   EF1α

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-DiO-eGFP-2A-mCherry_YDA

   Plasmid

   #194881

   Purpose

   Ratiometric YDA sensor for in vivo cre-dependent neuron-specific cholesterol visualization

   Depositor

   Peter Scheiffele

   Article

   Czernecki et al bioRxiv 2022.11.16.516849

   Insert

   GFP-T2A-mCherry-YDA
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PYPQ121-DdCBE

   Plasmid

   #218174

   Purpose

   Express plastid DdCBE, composed of TALE, split DddA originated from Burkholderia cenocepacia and UGIs

   Depositor

   Yiping Qi

   Article

   Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

   Insert

   TALE-Intron-N-DddAtox half (G1397N)-UGI-T2A-TALE-C-DddAtox half (G1397C)-UGI
   

   Use

   Plastid base editing

   Expression

   Plant

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TC562

   Plasmid

   #164887

   Purpose

   Punc-4::rtetR-QFAD(650-777+790-816)::P2A::mKate::T2A::tetR-pie1

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   QFAD mutated
   

   Expression

   Worm

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK591

   Plasmid

   #71589

   Purpose

   Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase
   

   Expression

   Bacterial

   Mutation

   changes threonine-2 to alanine and aspartate-177 …

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB-puro donor.dna

   Plasmid

   #181923

   Purpose

   attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attB-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Expression

   Mammalian

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-sgAAVS1-2

    Plasmid

    #129727

    Purpose

    Expressing SpCas9 and sgAAVS1-2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-2
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. attP-puro donor

    Plasmid

    #182139

    Purpose

    attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Expression

    Mammalian

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN(CMV-copGFP-Luc2-Puro)

    Plasmid

    #80389

    Purpose

    To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Luc2-F2A-Puromycin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458-mcherry

    Plasmid

    #161974

    Purpose

    Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Cas9-t2a-mcherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBMN(CMV-copGFP-Puro-SMAR)

    Plasmid

    #80391

    Purpose

    expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Puromycine
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn1-FLEX-CybGam-WPRE

    Plasmid

    #207662

    Purpose

    Control for CybSEP2

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Gamillus
    

    Use

    AAV

    Mutation

    Histidine 86 and 159 to alanines, inserted gamill…

    Promoter

    hSynapsin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Promoter

    tight TRE

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRL_U2AF1_WT_mCherry

    Plasmid

    #84017

    Purpose

    expression of U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSQT1601

    Plasmid

    #53369

    Purpose

    human codon optimized Csy4 and FokI-dCas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

    Plasmid

    #229791

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. piRFP

    Plasmid

    #31857

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Has Service

    Cloning Grade DNA

    Insert

    iRFP
    

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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