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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,264 results for: Vars

Showing: 1801 - 1820 of 11264 results

  1. pLKO.3G_X7

    Plasmid
    #171213
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites
    Depositor
    Jenny Wang
    Insert
    N/A; empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    171213-plasmid-map-sequence-id-338282

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  2. p6187

    Plasmid
    #198158
    Purpose
    bacterial expression of sifA::HaloTag fusion, mutant form
    Depositor
    Michael Hensel
    Insert
    PsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    198158-plasmid-map-sequence-id-386978

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  3. pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER

    Plasmid
    #52687
    Purpose
    Same as 41025, with additional linker
    Depositor
    Michael Nick Boddy
    Insert
    tetO7-Pcyc1-GFP+LINKER
    Use
    Promoter replacement
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52687-plasmid-map-sequence-id-82017

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  4. pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER

    Plasmid
    #52689
    Purpose
    Same as 41022, with additional linker
    Depositor
    Michael Nick Boddy
    Insert
    tetO7-Pcyc1-3xFLAG+LINKER
    Use
    Promoter replacement
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    52689-plasmid-map-sequence-id-82019

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  5. SnFR_S074

    Plasmid
    #182811
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S074-CholineSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182811-plasmid-map-sequence-id-358311

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  6. pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER

    Plasmid
    #52686
    Purpose
    Same as 41024, with additional linker
    Depositor
    Michael Nick Boddy
    Insert
    tetO7-Pcyc1-3xHA+LINKER
    Use
    Promoter replacement
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52686-plasmid-map-sequence-id-82016

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  7. SnFR_S062

    Plasmid
    #182810
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S062-CholineSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182810-plasmid-map-sequence-id-358310

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  8. pFA6a-kanMX-tetO-Pcyc1-GST-LINKER

    Plasmid
    #52688
    Purpose
    Same as 41026, with additional linker
    Depositor
    Michael Nick Boddy
    Insert
    tetO7-Pcyc1-GST+LINKER
    Use
    Promoter replacement
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    52688-plasmid-map-sequence-id-82018

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  9. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7.AviTag
    Use
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  10. pGMC00010 (aka pRC0495)

    Plasmid
    #195295
    Purpose
    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195295-plasmid-map-sequence-id-387224

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  11. pGMC00009 (aka pRC0494)

    Plasmid
    #195294
    Purpose
    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195294-plasmid-map-sequence-id-385588

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  12. SnFR_V.7.1.2

    Plasmid
    #182809
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    V.7.1.2-CholineSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    182809-plasmid-map-sequence-id-358174

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  15. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Promoter
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  16. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  18. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid
    #171938
    Purpose
    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins
    Depositor
    David Berson
    Insert
    Apex2, mRuby2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171938-plasmid-map-sequence-id-338493

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  19. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid
    #171937
    Purpose
    Expresses APEX2 linked to tau for labeling microtubules
    Depositor
    David Berson
    Insert
    tau-APEX2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171937-plasmid-map-sequence-id-338490

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  20. pLS1/-11

    Plasmid
    #31492
    Depositor
    Kathleen Matthews
    Insert
    Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 21, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 1801 - 1820 of 11264 results