We narrowed to 5,840 results for: pCas

1. pET-FLAG-eSpCas9-plus

   Plasmid

   #126774

   Purpose

   Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Osa-pCAMBIA-1301-STTM166

   Plasmid

   #84197

   Purpose

   Disturb the expression of miR166 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR166
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS/Daxx myc S669A

   Plasmid

   #19282

   Depositor

   Philip Leder

   Article

   Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

   Insert

   Daxx myc S669A (Daxx Mouse)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pBW820_pCAG-pExpr-FULL-ADDER

   Plasmid

   #87552

   Purpose

   Encodes full adder logic in response to Cre, Flp and VCre recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP, mCherry
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCaSpeR4 cyto Grx1-roGFP2

   Plasmid

   #64999

   Purpose

   Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Tags

   roGFP2

   Expression

   Insect

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-PB1-MontiRed-LOV2dark

   Plasmid

   #178874

   Purpose

   Mammalian expression of PB1-MontiRed-LOV2dark

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-MontiRed-LOV2dark
   

   Expression

   Mammalian

   Mutation

   LOV2: C450A, L514K, G528A, L531E, N538E

   Promoter

   CAG

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCA-T-intron(Neo)-G(FLLFLNL)

   Plasmid

   #40021

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (ATG)
   GFP
   beta-globin intron
   Neo
   

   Mutation

   deleted nucleotides 1-274 and insertion of FRT, …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available Since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCA-G-intron(Neo)-T(FLLFLNL)

   Plasmid

   #40020

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdT-3Myc
   beta-globin intron
   Neo
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available Since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMS7: pCascade(AvCAST)_entry

   Plasmid

   #168140

   Purpose

   Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-AaCas12b(D977A)-2AeGFP

    Plasmid

    #121962

    Purpose

    Mammalian expression, Transcription regulation, deactivated AaCas12b

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    Catalytically deactivated AaCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    D977A

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBT374_pattB-pCA-GFP-pA-attBSwa

    Plasmid

    #52553

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-MCP-VP64-2AmScarlet

    Plasmid

    #121963

    Purpose

    Mammalian expression, Transcription regulation module

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    VP64 module fused with MCP, recruited by MS2 hairpin
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-hHIF1 alpha-IGd2

    Plasmid

    #22535

    Purpose

    To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

    Plasmid

    #92433

    Purpose

    expresses Cas9 and gRNA targeting PAFAH1B1

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS/Daxx myc S502A

    Plasmid

    #19281

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    Daxx myc (Daxx Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-ChroME(F243Y)-mRuby2-ST

    Plasmid

    #109127

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(F243Y)-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 161+80pCalb2/mutCREB(-2)-like

    Plasmid

    #66751

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, mutant)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp +80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    GCGC (-2) to TAGA

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. -161+80pCalb2/mutNRF-1(-41)-like

    Plasmid

    #66744

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, mutant)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    CGC (-41) to ATT

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. -161+80pCalb2/dmutNRF-1(-41;-35)-like

    Plasmid

    #66741

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, mutant)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    CGCAGGCGC (-41;-35) to ATTAGGATT

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Chronos(M140T)-mRuby2-ST

    Plasmid

    #109129

    Purpose

    Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    Chronos(M140T)-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge