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pICSL86977OD:PITG_02860a

Plasmid

#173507

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_02860_P17777

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_00582

Plasmid

#173506

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_00582_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA3

Plasmid

#171001

Purpose

HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIV]

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT I261A

Plasmid

#153476

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT I261A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, I261A

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT S333A

Plasmid

#153477

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT S333A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, S333A

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin delta2step

Plasmid

#153479

Purpose

Mammalian expression of FLAG-tagged Tapasin delta2step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.13-14, 16-17

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin delta3step

Plasmid

#153480

Purpose

Mammalian expression of FLAG-tagged Tapasin delta3step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.12-14, 16-18

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pETCON-Aga2p-TAPBPR-myc

Plasmid

#153486

Purpose

Yeast expression of Aga2p-TAPBPR-myc

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

Aga2P and c-myc epitope tag

Expression

Yeast

Promoter

GAL

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT E102A

Plasmid

#153473

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT E102A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, E102A

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT M122A

Plasmid

#153474

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT M122A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, M122A

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT G212A

Plasmid

#153475

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT G212A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, G212A

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT

Plasmid

#141309

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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sgAAT Nicking

Plasmid

#169842

Purpose

Express a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutation

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

sgAAT Nicking (SERPINA1 Human)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ192Annexin A11

Plasmid

#164498

Purpose

For bacterial expression of rat His-tagged Δ192Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ192Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ188Annexin A11

Plasmid

#164497

Purpose

For bacterial expression of rat His-tagged Δ188Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ188Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-EGFP

Plasmid

#132974

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

eGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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563aa ELL2 pEF4B

Plasmid

#127273

Purpose

Expresses 563aa human WT ELL2

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 truncated at SphI (ELL2 Human)

Expression

Mammalian

Mutation

Contains 564 N-terminal amino acids ELL2, termina…

Promoter

pEF1a

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TtNAM-B1(1-215)

Plasmid

#124577

Purpose

NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Article

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TtNAM-B1 (1-215)

Use

Topo gateway cloning vector

Promoter

None

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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