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C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

Plasmid

#162918

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase/green fluorescent protein fusion with upstream IRES sequence

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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TB:2xP53_RE:MiniP

Plasmid

#133881

Purpose

2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

2 copies of the p53 DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV1_1-1009

Plasmid

#129471

Purpose

Mammalian expression of the N-terminus + central domain of bovine SV1

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

Supervillin (SVIL Bovine)

Expression

Mammalian

Mutation

contains amino acids 1-1009

Promoter

CMV

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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UAS-Xbp1-GFP

Plasmid

#80627

Purpose

expression of Xbp1-GFP (RA isoform) in Drosophila

Depositor

Pedro Domingos, Herman Steller

Article

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Tags

EGFP

Expression

Insect

Mutation

RA isoform

Promoter

UAS

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
STAGR_SAMScaffold_h7SK

Plasmid

#102846

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_h7SK

Use

Pcr template for stagr inserts

Promoter

H7SK

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Barkor Δ10

Plasmid

#30500

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

1-483aa

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
mCherry-SAC1-HLx8

Plasmid

#108139

Purpose

SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPD3C

Plasmid

#86971

Purpose

Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA3 fused to phosphite dehydrogenase and P450cam

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, N106R in PCNA3, silent mutat…

Promoter

T7 promoter

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2209

Plasmid

#36672

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HOXA13-TALEN_Right (HOXA13 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
cmamxA1-GFP

Plasmid

#107195

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A1

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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