165,173 results

1. pCMV6-AC EJ7-GFP

   Plasmid

   #113617

   Purpose

   Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   EJ7-GFP variant of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Artichoke

   Plasmid

   #73320

   Purpose

   Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and EF1a

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. RMCE halo-flag DNA-PKcs

   Plasmid

   #233918

   Purpose

   Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   flag-halo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

   Plasmid

   #192616

   Purpose

   AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   FLEX-RIboL1-jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1_hTRIM32

   Plasmid

   #69541

   Purpose

   Expresses human TRIM32 fused to c-terminal EGFP

   Depositor

   Martin Dorf

   Article

   Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

   Insert

   tripartite motif containing 32 (TRIM32 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-Tmem192-mRFP-3xHA

   Plasmid

   #134631

   Purpose

   bait for affinity-isolation of lysosomes

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   3xHA, TEV, and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TDP-43_CTD_M337V

   Plasmid

   #98674

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M337V

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA GSK3 beta S9A pcDNA3

   Plasmid

   #14754

   Purpose

   Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

   Depositor

   Jim Woodgett

   Article

   Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

   Insert

   GSK3 beta S9A (GSK3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S9A. Constitutively active mutant.

   Promoter

   Available since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pQFDBD-2x AD*-VP16*, α-cry:EGFP

   Plasmid

   #180473

   Purpose

   Gene expression vector pQFDBD-2x AD*-VP16*, α-cry:EGFP

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Insert

   QFDBD-2xQFAD*-VP16*
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP1427 - pAAV-AiE2135m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214497

    Purpose

    AiE2135m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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