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HybridF-IRES-tauGFP LNL TV

Plasmid

#15654

Depositor

Peter Mombaerts

Publication

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

HybridF-IRES-tauGFP LNL TV

Use

High copy number

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut G

Plasmid

#11320

Depositor

Phil Sharp

Publication

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation G (see paper) on inner 2 binding sites.

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 C

Plasmid

#11326

Depositor

Phil Sharp

Publication

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation C from figure 4A of paper.

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 I

Plasmid

#11332

Depositor

Phil Sharp

Publication

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation I from figure 4A of paper.

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-2T-M

Plasmid

#1128

Depositor

Susan Lindquist

Publication

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

M (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmEDC3

Plasmid

#21713

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Enhancer of decapping 3 (Edc3 Fly)

Use

Drosophila expression

Tags

EGFP

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral

Plasmid

#21722

Depositor

Elisa Izaurralde

Publication

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

EGFP

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-cGAS-HA

Plasmid

#231895

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Publication

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Tags

3xHA and mCherry

Expression

Mammalian

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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plk1 WT GFP-myc

Plasmid

#132963

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Publication

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Publication

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ1208

Plasmid

#44490

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]

Use

Gateway expression clone

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cx36-GCaMP

Plasmid

#123604

Purpose

Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

Depositor

John O'Brien

Publication

Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

Insert

Connexin 36-GCaMP3 fusion (Gjd2 Mouse)

Tags

GCaMP3

Expression

Mammalian

Promoter

CMV

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His-Keap1-Tev-Halo

Plasmid

#87545

Purpose

expresses C-terminal Halo-tagged human Keap1 in mammalian cells

Depositor

Yimon Aye

Publication

Parvez et al Nat Protoc. 2016 Dec;11(12):2328-2356. d

Insert

Keap1 (KEAP1 Human)

Tags

Halo and His-Tag

Expression

Mammalian

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCis_2+7+8+5

Plasmid

#60589

Purpose

a 4-integrase plasmid

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+7+8+5

Expression

Bacterial

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform IV

Plasmid

#120320

Purpose

Septin 11 isoform IV mammalian expression

Depositor

Angel de Blas

Publication

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA7-mRuby3

Plasmid

#184893

Purpose

Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Publication

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA7 (ANXA7 Human)

Tags

mRuby3

Expression

Mammalian

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_TEV

Plasmid

#58872

Purpose

MESA protease chain with dTomato ectodomain and no linker domain

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with dTomato ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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1x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64390

Purpose

encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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