We narrowed to 4,244 results for: Pet2;

1. pET28-His8-aCat(660-885)

   Plasmid

   #203334

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-885 only

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a His6 GST PYL2(14-188)

   Plasmid

   #177842

   Purpose

   Bacterial Expression of PYL2

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 2 (PYL2 Mustard Weed)
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Fgβ-anticalin-His-ybbr

   Plasmid

   #168040

   Purpose

   Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag, Fgβ peptide, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-Avi-Coh (E154C)-HIS

   Plasmid

   #153447

   Purpose

   E. coli expression of Rc. CohE construct with E154C mutation.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.CohE
   

   Use

   Tags

   6xHis and Avi-tag

   Expression

   Bacterial

   Mutation

   E154C mutation on CohE

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SznF H407A H409A H448A

   Plasmid

   #139566

   Purpose

   expressed SznF H407A H409A H448A in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF H407A H409A H448A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 215 to alanine

   Promoter

   T7

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pet28a His6-HSPB7 (zebrafish) C49S

   Plasmid

   #110071

   Purpose

   Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   His6-HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Cysteine 49 to Serine

   Promoter

   T7

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-FFSARG-ddFLN4-HIS-ybbr

   Plasmid

   #101240

   Purpose

   minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FFSARG peptide, ddFLN4 fingerprint
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-Hook3 Q147A, M151A, I154A

   Plasmid

   #74616

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Q147A, M151A, I154A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-Hook3 N68A, W69A, K77A

   Plasmid

   #74619

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-ybbR-HIS-Spec(x2)-DocI

    Plasmid

    #58713

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Spec(x2)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b(+)_S. cerevisiae Hbs1(162-611)

    Plasmid

    #39337

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Pet28a His6-HSPB7 (zebrafish) C117S

    Plasmid

    #110072

    Purpose

    Express C117S mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 117 to Serine

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. Kv7.4 BD (522-645, C643A)/pET28HMT

    Plasmid

    #111073

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 BD (522-645, C643A)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

    Plasmid

    #117981

    Purpose

    SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B1
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

    Plasmid

    #117982

    Purpose

    SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B2
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

    Plasmid

    #117983

    Purpose

    SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_B2
    

    Use

    Tags

    HIS, sortase, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-6xHis-MBP-TEV site-TadA8r

    Plasmid

    #208318

    Purpose

    Overxpression MBP-TadA8r

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET264-pUC 24xMS2V6 Loxp KANr Loxp

    Plasmid

    #104393

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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