We narrowed to 9,419 results for: plat

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Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6011nes

Plasmid

#108652

Purpose

An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-ERK-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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EP-Pol vector

Plasmid

#131686

Purpose

A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Mutation

F421Y, D827A

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-CMV-GFP-BGHpA

Plasmid

#190239

Purpose

Expresses EGFP in mammalian cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-1

Plasmid

#163127

Purpose

pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Tags

Ubiquitin

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Bs-1

Plasmid

#163137

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-UnaG-P2A-FusionRed

Plasmid

#191109

Purpose

Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

UnaG

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA

Plasmid

#216229

Purpose

S. aureus USA300 GyrA Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

Plasmid

#115209

Purpose

Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PbuCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJAT71

Plasmid

#204305

Purpose

Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-DsRed

Use

CRISPR

Promoter

ie1

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Wild-type AdPol vector

Plasmid

#131685

Purpose

A lentiviral vector that constitutively expresses the wild-type adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Available Since

June 27, 2020

Availability

Academic Institutions and Nonprofits only

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KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

Plasmid

#115216

Purpose

Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin2Cas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Multisite gateway entry clone

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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