We narrowed to 29,077 results for: ple

1. pLEX-FLAG-NRAS-IRES-Puro

   Plasmid

   #120574

   Purpose

   Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII-Topo Plexin D1 in situ probe

   Plasmid

   #45621

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

   Insert

   Plexin D1 in situ probe (Plxnd1 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 5751-6406 of NM_026376.3

   Promoter

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pLentiPGK Puro DEST JNKKTR AE Clover

   Plasmid

   #90240

   Purpose

   Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-RAP1A-E63-neo

   Plasmid

   #156174

   Purpose

   Lentiviral vector for expression of RAP1A-E63, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Glutamine 63 to Glutamic acid

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF1a-GFP-miRE_shRen-Blast

   Plasmid

   #135682

   Purpose

   Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-UbcP-HA-YWAA-pGK-HYG

   Plasmid

   #107379

   Purpose

   Lentiviral expression of HA-YWAA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   CRBN-Y383A/W385A

   Promoter

   UbcP

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

   Plasmid

   #55199

   Purpose

   CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   ECFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CD74-ROS1 S1986F/G2032R

   Plasmid

   #183821

   Purpose

   Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Mutation

   S1986F/G2032R

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

   Plasmid

   #69833

   Purpose

   Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyTag-C1C2
   

   Use

   Lentiviral

   Tags

   Trafficking signal and mCherry

   Expression

   Mammalian

   Mutation

   Inserted SpyTag after the signal peptide of C1C2.

   Promoter

   CamKIIa

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-X1-Neo-GFP-ATL1-G20TM

    Plasmid

    #109019

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-deltaTM (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    truncated form without C-terminal ER transmembran…

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-HCoV-NL63-3CL-C144A

    Plasmid

    #160287

    Purpose

    Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C144A

    Promoter

    EF1a

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-X1-Neo-GFP-ATL2-R244Q

    Plasmid

    #109023

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-R244Q (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Arg244Gln mutation results in dimerization defect

    Promoter

    EIF-1a promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti X2 Zeo/pTER shGFP #2 (790-1)

    Plasmid

    #22529

    Depositor

    Eric Campeau

    Insert

    sh Enhanced Green Fluorescent Protein #2
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX307-SARS-CoV-3CL-C145A

    Plasmid

    #160281

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EF1a-MTS-FLAG-LOV*

    Plasmid

    #202208

    Purpose

    Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    

    Use

    Lentiviral

    Tags

    FLAG and LOV*

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti X2 Hygro/pTER shLUC (w607-1)

    Plasmid

    #20958

    Purpose

    3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Hygro

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-X1-Neo-GFP-ATL2-deltaTM

    Plasmid

    #109021

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-deltaTM (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    truncated form without C-terminal ER transmembran…

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX-MAPKAP1.1-FHH-IRES-Puro

    Plasmid

    #120705

    Purpose

    Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 Isoform 1
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PGK-RAP2A-V12-neo

    Plasmid

    #156178

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CD74-ROS1 S1986F/L2000V

    Plasmid

    #183818

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2000V mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    S1986F/L2000V

    Promoter

    CMV

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge