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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51

Plasmid

#33230

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pMG13

Plasmid

#122932

Purpose

Expression of the regulator tTA.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

tTA

Expression

Yeast

Promoter

Pcln3

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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ClhN-4V5-APEX2-URA

Plasmid

#207056

Purpose

For ORF tagging with 4V5-APEX2

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Tags

4xV5-APEX2

Expression

Yeast

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMM0531

Plasmid

#128988

Purpose

Part plasmid for VP16-CIB1, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-VP16-CIB1

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0528

Plasmid

#128985

Purpose

Part plasmid for pZF(3BS), Type 2 (promoter) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTCW002

Plasmid

#83522

Purpose

pheromone mediated destablised-GFP expression in yeast

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-yEGFPCLN2PEST

Expression

Yeast

Promoter

pFUS1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-101

Plasmid

#111307

Purpose

prp8-101

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-101 (E1960K)

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-Sad1

Plasmid

#90297

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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p414TEF_Hsp82_CycTER

Plasmid

#61711

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pWZ414-F53

Plasmid

#23077

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F30

Plasmid

#23066

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 9 to Glutamine H3 K9Q

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pET28b-sad1-C63A

Plasmid

#90302

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

C63A

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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YEplac181-GAL11

Plasmid

#8628

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

GAL11 (GAL11 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51 KR

Plasmid

#33232

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Mutation

K191R

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
CDC12(K351N)

Plasmid

#1163

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

K351N

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(K351N)

Plasmid

#1166

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Mutation

K351N

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(E368Q)

Plasmid

#1167

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

E368Q

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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