We narrowed to 4,244 results for: Pet2;

1. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

   Plasmid

   #235150

   Purpose

   Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   HaloTag linked to SpyCatcher3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-His-MBP-NLS-RfxCas13d-NLS-HA

   Plasmid

   #171586

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS-HA

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   His-MBP-NLS-RfxCas13d-NLS-HA
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a human Tankyrase2 full length Y920E

   Plasmid

   #132639

   Purpose

   expresses human Tankyrase2 full length Y920E in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

   Insert

   Tankyrase2 Y920E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET251-pUC 12xMS2V6 Loxp KANr Loxp

   Plasmid

   #104392

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

   Plasmid

   #181744

   Purpose

   bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   bacterial codon optimized SpRY-HF1
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS(SV40)-6xHis and MKIEE MBP tag

   Expression

   Bacterial

   Mutation

   SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…

   Promoter

   T7

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-6xHis-MBP-TEV site-TadA8e

   Plasmid

   #208319

   Purpose

   Overxpression MBP-TadA8e

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8e
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-His6-SUMO-cSrc-SH2-AviTag

   Plasmid

   #214230

   Purpose

   Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

   Insert

   cSrc kinase SH2 domain (SRC Human)
   

   Use

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

   Plasmid

   #171587

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS 6His

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   MKIEE-NLS-RfxCas13d-NLS-HA-6His
   

   Use

   Tags

   HA-6HIS and MKIEE

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid

    #177335

    Purpose

    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pET28a-GGG-HIS-CBM-Xmod-DocIII

    Plasmid

    #91698

    Purpose

    CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-HIS-CBM-Xmod-DocIII
    

    Use

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. His6-Twin-Strep-SUMO-pET28a-EfaCas2

    Plasmid

    #112794

    Purpose

    To express enterococcus faecalis Cas2 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas2
    

    Use

    Tags

    His6-Twin-Strep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPV00554 pET21GG2-His(6)-BECN(248-450)

    Plasmid

    #136685

    Purpose

    Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 ECD/BARA (BECN1 Human)
    

    Use

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FabF (KASII) pet28 N-terminal His Tag

    Plasmid

    #75017

    Purpose

    Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

    Depositor

    Michael Burkart

    Article

    Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

    Insert

    FabF (fabF E. coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-CTLA4-FLN-ELP-His-ybbr

    Plasmid

    #168038

    Purpose

    Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
    

    Use

    Tags

    3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pET28b – E. coli RecE residues 564-868

    Plasmid

    #104534

    Purpose

    Expresses E. coli RecE residues 564-868

    Depositor

    Charles Bell

    Article

    Zhang et al Structure. 2009 May 13;17(5):690-702. do

    Insert

    RecE residues 564-866 (C-terminal nuclease domain)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21 Catalytic Domain of MALT1 Catalytic Mutant

    Plasmid

    #48971

    Purpose

    Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 (MALT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains AA 329-566; Catalytic Mutant C464A

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175784

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Use

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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