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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,079 results for: nes

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Showing: 1841 - 1860 of 16079 results

  1. pGTM_AviHTSumoTOEET_NESG-HRV2-3CLpro

    Plasmid
    #254122
    Purpose
    Expresses HRV2 3CLpro in E. coli with fused Avi-tag, 6xHis and Sumo SET tag
    Depositor
    Gaetano Montelione
    Insert
    3C protease
    Tags
    Avi-tag, 6xHis, TEV site, Sumo
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    254122-plasmid-map-sequence-id-505750

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  2. pUC19_21 tRNA genes

    Plasmid
    #242430
    Purpose
    In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.
    Depositor
    Sebastian Maerkl
    Article
    Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:
    Insert
    21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
    Expression
    Bacterial
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242430-plasmid-map-sequence-id-485004

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  6. mGeos2-VEL-Farnesyl-5

    Plasmid
    #57548
    Purpose
    Localization: Membrane, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mGeos2-VEL
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57548-plasmid-map-sequence-id-218618

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  17. pCMV-MMLV-gag-hMLH1dn

    Plasmid
    #195513
    Purpose
    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.
    Depositor
    Hyongbum Kim
    Article
    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.
    Insert
    hMLH1dn (MLH1 Human)
    Use
    Virus like particle
    Tags
    FLAG, NES, and Protease cleavate site
    Expression
    Mammalian
    Mutation
    Human MLH1 del754-756
    Promoter
    CMV
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195513-plasmid-map-sequence-id-384690

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  18. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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  20. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107303
    Purpose
    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS2
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107303-plasmid-map-sequence-id-204639

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Showing: 1841 - 1860 of 16079 results