We narrowed to 7,267 results for: trac

1. pRS416gT-GalL-Cas9

   Plasmid

   #79903

   Purpose

   pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

   Depositor

   Ronald Davis

   Article

   Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

   Inserts

   Cas9
   guide RNA (gRNA)
   Tetracycline Repressor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPM1 promoter, GalL, and RPR1 promoter with TetO …

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM270 - MCS mosTI hygroR target

   Plasmid

   #159826

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI hygroR target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ2370 - intron GG3 - 250bp

   Plasmid

   #159879

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG3 - 250bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SNV-gfp-IRES-MCS

   Plasmid

   #53122

   Purpose

   Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

   Depositor

   Jeanette Hyer

   Article

   Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

   Type

   Empty backbone

   Use

   Retroviral; For expression in chicken cells

   Tags

   EGFP-IRES

   Expression

   Mutation

   Promoter

   SNV (spleen necrosis virus)

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-EF1a-GFP ArgSen

   Plasmid

   #184695

   Purpose

   Lentiviral transfer plasmid for expression of GFP ArgSen

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   GFP ArgSen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCB6HindIII-rat FcRn

   Plasmid

   #121671

   Purpose

   Expresses rat neonatal Fc receptor in mammalian cells

   Depositor

   Pamela Bjorkman

   Article

   Tesar et al Traffic. 2006 Sep;7(9):1127-42.

   Insert

   rat neonatal Fc receptor (Fcgrt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a - APX

   Plasmid

   #89618

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Ascorbate dependent Tryparedoxin peroxidase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXGFPC-5 Pxyl-dcas9 (Spa)

   Plasmid

   #133318

   Purpose

   expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pasteurianus)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. NpT7-5-ERK1

   Plasmid

   #39229

   Depositor

   Melanie Cobb

   Article

   Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

   Insert

   ERK1 (MAPK3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pB80-iLID-mCherry-RAB5

    Plasmid

    #174624

    Purpose

    Optogenetic coupling to early endosomes (RAB5) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    iLID-mCherry-RAB5 (RAB5A Synthetic, Human)
    

    Use

    Tags

    iLID-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del; RAB5: Met1Del

    Promoter

    Chicken beta-actin

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. tol2-mpx-lyn-GFP

    Plasmid

    #35037

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    Lyn-GFP (lyn Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

13. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-3GFP-kanMX6

    Plasmid

    #105148

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, Thomas Pollard, David Kovar

    Article

    Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

    Insert

    3GFP-kanMX6
    

    Use

    Tags

    3GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fast-GCaMP-EF05

    Plasmid

    #52558

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF05
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed from GCaMP3: D397N/G398A/N399D

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pWKS1583

    Plasmid

    #70188

    Purpose

    Modular vector for secreted amylase reporter expression

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

    Insert

    amyEopt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry Tensin2

    Plasmid

    #105300

    Purpose

    To study localization and function of Tensin 2 with EGFP Tensin1

    Depositor

    Kenneth Yamada

    Article

    Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

    Insert

    Tensin2 (TNS2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    lacks N-terminal C1 domain

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM41-EcPPXc

    Plasmid

    #38329

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pGAN101

    Plasmid

    #131123

    Purpose

    Plasmid for overexpression of prion domains to induce prions

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    mKate2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKM263-EcPPXc

    Plasmid

    #38328

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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