We narrowed to 2,325 results for: tub
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Plasmid#69115PurposeDual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationDeletion of 678-681aa region. Silent mutations i…PromoterCMVAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only
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Str-KDEL_SBP-EGFP-CD59
Plasmid#222307PurposeSynchronize the trafficking of CD59 from the ER.DepositorInsertStreptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)
UseTagsExpressionMammalianMutationsignal peptide removedPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP
Plasmid#212106PurposeExpression of GABARAP split tandem protein with mTagBFP2DepositorInsertGABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)
UseTagsmTagBFP2ExpressionMammalianMutationG116A (lipidation deficiency) of upstream GABARAPPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)
Plasmid#212108PurposeExpression of lipidation deficient GABARAP split tandem protein with mTagBFP2DepositorInsertGABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Entacmaea quadricolor, Human)
UseTagsmTagBFP2ExpressionMammalianMutationG116A (lipidation deficiency) of both GABARAPsPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
K365-iLid
Plasmid#188456PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis and iLidExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mApple-LZ-LOV2
Plasmid#197418PurposeMammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD
Plasmid#196881PurposeNeuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.DepositorInsertmOrange2-Cdk5rap2-CTD
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB
Plasmid#193719PurposeRapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transportDepositorInserthsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
UseTags2xHA-FRBExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T
Plasmid#191331PurposeExpresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast
Plasmid#182887PurposeTransfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsEGFPExpressionBacterial and MammalianMutationsilet mutations in aa 197 - 203 to confer resista…PromoterEF1alpha coreAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EGFP-P2A_truncHaus6_IRES_Blast
Plasmid#182885PurposeTransfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsExpressionBacterial and MammalianMutationstop codon introduced 2 amino acids downstream of…PromoterEF1alpha coreAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep
Plasmid#180314Purposebacterial co-expression of human SEPT7 and of human SEPT9_i1-i5DepositorUseTagsTEV-StrepExpressionBacterialMutationSEPT9_i1-i5PromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 S22A S23A-msfGFP
Plasmid#180338Purposemammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 S22A S23APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 R10A R15A-msfGFP
Plasmid#180336Purposemammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 R10A R15APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 S12A S13A-msfGFP
Plasmid#180337Purposemammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 S12A S13APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
SITS-EGFP-Bin1b
Plasmid#176021PurposeLeishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRSDepositorInsertBin1b (bin1b Zebrafish)
UseLeishmania cell free expressionTagsEGFPExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cavin4a-mCherry
Plasmid#176010PurposeMammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYMDepositorInsertCavin4a (cavin4a Zebrafish)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP
Plasmid#108503Purposeinducible expression of vsv-INCENP d543-746-EGFPDepositorInsertINCENP d543-746 (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationdelta alpha helixPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP
Plasmid#108505Purposeinducible expression of vsv-INCENP 8KR-EGFPDepositorInsertINCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationK548R, K563R, K602R, K609R, K614R, K622R, K630R, …PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only