165,191 results

1. pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE

   Plasmid

   #183523

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   rsChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I146M/G174S

   Promoter

   CaMKIIa

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP12157 - pAAV-AiE0395h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2157)

   Plasmid

   #208146

   Purpose

   Direct-expressing SYFP2 in oligodendrocytes AAV Virus

   Depositor

   Boaz Levi, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS-CoV2 Nucleocapsid

   Plasmid

   #153201

   Purpose

   Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

   Depositor

   Peter Klein

   Article

   Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
   

   Use

   Tags

   Myc tag (5X)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. dRED

   Plasmid

   #139836

   Purpose

   Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RNase H1 protein contains D210N mutation. dCas9 …

   Promoter

   tet-inducible CMV

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-RH

   Plasmid

   #60516

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDe-CAS9-D10A

   Plasmid

   #61477

   Purpose

   based on pDe-CAS9, encodes Cas9 D10A nickase variant

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   catalytic Asp10 changed to Ala

   Promoter

   PcUbi4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS2115

   Plasmid

   #49140

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   ssAAV-Ple155-emGFP-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pKb-TnpB1

   Plasmid

   #215333

   Purpose

   ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

   Depositor

   Kutubuddin Molla

   Article

   Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

   Insert

   Rice codon optimized ISDra2 TnpB
   

   Use

   Tags

   Not applicable

   Expression

   Plant

   Mutation

   Promoter

   Rice ubiquitin promoter for TnpB and OsU3 promote…

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV8)

   Viral Prep

   #87306-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Promoter

   EF1a

   Tags

   None

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pMXSIP-FLAG-ATPIF1

    Plasmid

    #204703

    Purpose

    expresses FLAG-ATPIF1 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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