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pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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BSP608

Plasmid

#122253

Purpose

expression vector for mNeptune under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mNeptune

Expression

Worm

Promoter

EFT3

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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MAPTW

Plasmid

#205695

Purpose

shRNAmir backbone for RNA interference, with mTagBFP2 reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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BSP607

Plasmid

#122252

Purpose

expression vector for mKO2 under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mKO2

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos3_AmpR

Plasmid

#119146

Purpose

Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos7_AmpR

Plasmid

#119150

Purpose

Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos5_AmpR

Plasmid

#119148

Purpose

Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW

Plasmid

#133368

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCF710

Plasmid

#121892

Purpose

EF1a-WNV-protease-mTagBFP2

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

EF1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLC_pFa

Plasmid

#114219

Purpose

POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-gap16-ClopHensor

Plasmid

#26006

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

gap16-ClopHensor

Tags

Gap16 and Strep-II

Expression

Mammalian

Mutation

N-term (16AA) of Gap-43

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pCF713

Plasmid

#121665

Purpose

U6-sgRNA EF1a-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4L5

Plasmid

#153156

Purpose

Expression of Flag tagged human FOXD4L5 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4L5 (FOXD4L5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE98

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW

Plasmid

#133379

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-LC-Myosin-C-10

Plasmid

#54145

Purpose

Localization: Light Chain Myosin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LC-Myosin

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
BII-CBtW

Plasmid

#133372

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pIM35

Plasmid

#74644

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

MET25

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCF709

Plasmid

#121891

Purpose

EF1a-ZIKV-protease-mTagBFP2

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

EF1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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