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AAV2-hSyn-G-Flamp1

Plasmid

#188569

Purpose

green biosensor for cAMP in neurons

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1

Use

AAV

Tags

His tag

Promoter

hSyn

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPPAT-2XStrep

Plasmid

#125620

Purpose

expresses human PPAT protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PPAT (PPAT Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mScarlet

Plasmid

#131001

Purpose

AAV vector to drive the expression of mScarlet under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV8

Insert

mScarlet

Use

AAV

Promoter

hSyn

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4 5HT3 LC IRES GFP

Plasmid

#123066

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L131G Q139L Y217F

Promoter

CAG

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138132

Purpose

Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP.90 beta

Plasmid

#108221

Purpose

Mammalian expression vector for EGFP fusion to human Hsp90 beta

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EGFP-Hsp90b (HSP90AB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#69496

Purpose

pJL1 expression vector with sfGFP

Depositor

Michael Jewett

Article

Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

Insert

Super folder GFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin WT

Plasmid

#78295

Purpose

Mammalian expression of mouse cofilin fused to EGFP

Depositor

Michael Frotscher

Article

Chai et al Development. 2016 Mar 15;143(6):1029-40.

Insert

cofilin 1 (Cfl1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin-S3D

Plasmid

#78297

Purpose

Mammalian expression of mouse cofilin S3D fused to EGFP

Depositor

Michael Frotscher

Article

Chai et al Development. 2016 Mar 15;143(6):1029-40.

Insert

cofilin 1 (Cfl1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

S3D

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCherry.90 beta

Plasmid

#108223

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 beta

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

mCherry-Hsp90b (HSP90AB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp1-mut

Plasmid

#188568

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138128

Purpose

Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

Plasmid

#176704

Purpose

neural activity dependent release of sbGLuc from presynaptic vesicles

Depositor

Ute Hochgeschwender

Article

Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

Inserts

Gaussia luciferase, slow burn
dTomato

Use

AAV

Tags

P2A and hPOMC1-26

Promoter

hSyn

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/Ecad-tdTomato

Plasmid

#101278

Purpose

Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

tdtomato

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

Plasmid

#194683

Purpose

CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-cyclin E

Plasmid

#216508

Purpose

Expresses cyclin E1 in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CCNE1 (CCNE1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2-hSyn-G-Flamp1-mut

Plasmid

#188570

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Use

AAV

Tags

His tag

Promoter

hSyn

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-Arch-GFP

Plasmid

#22222

Purpose

Cre-dependent viral expression of Arch-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Has Service

AAV1 and AAV9

Insert

Arch

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

no

Promoter

CAG

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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