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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,128 results for: try

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Showing: 1881 - 1900 of 3128 results

  1. pCDF-casBCDE(+6)

    Plasmid
    #89728
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Publication
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a longer versi…
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89728-plasmid-map-sequence-id-177602

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  6. pUAS-kkv-mos::NG

    Plasmid
    #138958
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4
    Depositor
    Paul Adler
    Publication
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    8 bp that are in the region that encodes the cata…
    Promoter
    UAS
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    138958-plasmid-map-sequence-id-274779

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  8. mCherry-hPMCA2xb

    Plasmid
    #47751
    Purpose
    mammalian expression of mCherry tagged hPMCA2, xb splice variant
    Depositor
    Emanuel Strehler
    Publication
    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PMCA2x/b (ATP2B2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    xb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pBad-CAts TAG97

    Plasmid
    #105668
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 97
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105668-plasmid-map-sequence-id-205515

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  13. SHIP2-ΔPS

    Plasmid
    #214901
    Purpose
    expression of the truncated variants of the SHIP2 without phosphatase domain
    Depositor
    Pavel Krejčí
    Publication
    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human SHIP2 delta aa 419-739 (INPPL1 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    delta 419-739aa
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    214901-plasmid-map-sequence-id-426068

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  16. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Publication
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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  17. pN1-mCherry-NKD2

    Plasmid
    #176641
    Purpose
    Expresses NKD2-mCherry fusion protein in mammalian cells
    Depositor
    Sonal Srikanth
    Publication
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Tags
    mCherry tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176641-plasmid-map-sequence-id-349875

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  18. pYPQ239-RVR

    Plasmid
    #108863
    Purpose
    FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R, K629R and N633R, Cpf1 is rice codon optimi…
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108863-plasmid-map-sequence-id-274167

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Showing: 1881 - 1900 of 3128 results