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pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52543

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐mTim1 (no tag)

Plasmid

#49206

Purpose

expresses mouse Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (Havcr1 Mouse)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
P6598 MSCV-IP N-HAonly TBPL1

Plasmid

#34896

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

TBPL1 (TBPL1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52547

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71345

Purpose

Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

hOTC_Cyan Nano-lantern(Ca2+)

Expression

Mammalian

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pCMV4-Flag-mIL-17RA/CFP.Hygro

Plasmid

#46861

Purpose

encodes Flag-tagged mouse IL-17RA fused to Cyan Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-CFP

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pAP2050-2

Plasmid

#105243

Purpose

mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pZF-AAA

Plasmid

#127465

Purpose

coding sequence for wild type zinc finger ZF-AAA (ZiF268)

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AAA (EGR1 Human)

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAP2042-1

Plasmid

#105244

Purpose

mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

Plasmid

#52540

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-VSFP2.3 rev. with Flex

Plasmid

#87948

Purpose

Plasmid used (after linearization) to generate transgenic mice that express the genetically encoded voltage indicator upon Cre recombination.

Depositor

Thomas Knopfel

Article

Quinn et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Voltage-Sensitive Fluorescent Protein 2.3

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S70A

Plasmid

#13620

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 70 to Ala

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Tags

His6-tev

Expression

Insect

Mutation

K70A

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL(T56A,DE)

Plasmid

#25059

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56D,DE)

Plasmid

#25060

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid changed le…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
mKO-Tubulin-6

Plasmid

#57850

Purpose

Localization: Microtubules, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Tubulin (TUBA1B Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52542

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1_OCT4_T234E_S235E

Plasmid

#40635

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

T234E_S235E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234E

Plasmid

#40636

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

T234E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234A

Plasmid

#40637

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Mutation

T234A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_S235E

Plasmid

#40638

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, and Thrombin Cleavage

Expression

Bacterial

Mutation

S235E

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_S235A

Plasmid

#40639

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…

Expression

Bacterial

Mutation

S235A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
3625 pSP72 Bim (genomic)

Plasmid

#8805

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

Insert

Bim genomic (Bcl2l11 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
3226 pSFFV-neo Bad S112A S136A

Plasmid

#8760

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S112A, S136A

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G13-L

Plasmid

#25762

Purpose

Entry vector with TRE promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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FLAG-ELL 1-373 pBacPAK8

Plasmid

#15328

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 1-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-621 pBacPAK8

Plasmid

#15329

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-621

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-373 pBacPAK8

Plasmid

#15330

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG ELL 500-621 pBacPAK8

Plasmid

#15331

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 500-621 only

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFUW-Syb2-R56X

Plasmid

#248137

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with R56X mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-R56X (Vamp2 Rat)

Expression

Mammalian

Mutation

R56X

Promoter

Ubiquitin

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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