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pcDNA3.1-mPCSK9

Plasmid

#122678

Purpose

Expresses mouse PCSK9 in mammalian cells

Depositor

Liming Pei

Publication

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 9 (Pcsk9) (Pcsk9 )

Expression

Mammalian

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-TRAIL-R2

Plasmid

#61383

Purpose

Expresses TRAIL receptor 2

Depositor

Francis Chan

Publication

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 2 (TNFRSF10B Human)

Tags

none

Expression

Mammalian

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCIG PIK3CA Wildtype

Plasmid

#73056

Purpose

Human PIK3CA Wildtype

Depositor

Joseph Gleeson

Publication

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

PIK3CA Wildtype (PIK3CA Human)

Expression

Mammalian

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET 28 full-length human PARP1

Plasmid

#192635

Purpose

Expresses human PARP1 with an N-terminal His-tag

Depositor

John Pascal

Publication

Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

Insert

Human PARP1 (PARP1 Human)

Tags

histidine-tag

Expression

Bacterial

Mutation

Polymorphism A762

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFX-mCherry-EEA1

Plasmid

#174452

Purpose

Express mCherry with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with early endosome-targeting sequence

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFX-mito-EGFP

Plasmid

#159096

Purpose

Express EGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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p55-H2B-Dendra2

Plasmid

#80609

Purpose

Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

Depositor

Periklis Pantazis

Publication

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

H2B-Dendra2 (H2BC21 Human, Synthetic)

Tags

Dendra2

Expression

Mammalian

Promoter

CAG

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pmAmetrine1.2

Plasmid

#42171

Depositor

Robert Campbell

Publication

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

mAmetrine1.2

Tags

6His

Expression

Bacterial

Promoter

araBAD

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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G protein alpha-q-GFP

Plasmid

#66080

Purpose

This G protein alpha-q construct contains internal insertions of GFP and the EE epitope

Depositor

Catherine Berlot

Publication

Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

Insert

G protein alpha-q-EE-YFP (Gnaq Mouse)

Tags

GFP was inserted internally into the alpha-q sequ…

Expression

Mammalian

Mutation

BamHI and SacI sites in alpha-q were removed with…

Promoter

CMV

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFX-Tom20-ｍCherry

Plasmid

#174188

Purpose

Express mCherry with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

Plasmid

#110558

Purpose

miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

Depositor

Joanna Goldberg

Publication

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

lacIq-Ptac

Expression

Bacterial

Promoter

lacIq-Ptac

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLHCX-Flag-mPKM1

Plasmid

#42511

Depositor

Dimitrios Anastasiou

Publication

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M1

Use

Retroviral

Tags

Flag

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGLO-GFP-1UAG

Plasmid

#82500

Purpose

Expresses GFP with 1 UAG codon at the amino acid position 3

Depositor

Chang Liu

Publication

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescent Protein with 1 UAG codon

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3

Promoter

AraBAD

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132399

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2(C358S)

Plasmid

#42513

Depositor

Dimitrios Anastasiou

Publication

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Cys358->Ser

Use

Retroviral

Tags

Flag

Mutation

Changed Cysteine 358 to Serine

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-HA-Ebp1

Plasmid

#67792

Purpose

Mammalian expression of Ebp1 tagged with HA

Depositor

Stephen Goff

Publication

Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

Insert

Ebp1 (PA2G4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
hOT-GFP

Plasmid

#67848

Purpose

mammalian expression of human oxytocin receptor with GFP tag

Depositor

Christian Gruber

Publication

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

oxytocin receptor (OXTR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
LentiGuidPuro-hTP53_sgRNA-1

Plasmid

#88853

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Publication

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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Mito-mGreenLantern

Plasmid

#164461

Purpose

Localization of mGreenLantern fluorescent protein to mitochondria

Depositor

Gregory Petsko

Publication

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-tMCK-ER-TurboID

Plasmid

#149412

Purpose

Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

tMCK

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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