Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,400 results for: fas

Clear keywords
Showing: 1901 - 1920 of 2400 results

  1. Halo-WIPI2 HRD

    Plasmid
    #207544
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells
    Depositor
    Jens Schmidt
    Publication
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag flanked by human WIPI2 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207544-plasmid-map-sequence-id-429537

    Enlarge

  3. pFBD-BirA_RFWD3:425-774

    Plasmid
    #210884
    Purpose
    Insect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    RFWD3:S425-E774 (RFWD3 Human)
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210884-plasmid-map-sequence-id-424500

    Enlarge

  4. pcDNA5/FRT/TO_Lyn11-STf

    Plasmid
    #182008
    Purpose
    CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines
    Depositor
    Kai Johnsson
    Publication
    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn11-STf
    Expression
    Mammalian
    Mutation
    Fast mutation on SNAP-tag E30R
    Promoter
    CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    182008-plasmid-map-sequence-id-355973

    Enlarge

  8. R-Q111R-N122D

    Plasmid
    #167178
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Publication
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-R-Q111R-N122D
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed R at 111 to Q and changed D at 122 to N
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167178-plasmid-map-sequence-id-334483

    Enlarge

  11. pNB784

    Plasmid
    #60163
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60163-plasmid-map-sequence-id-96372

    Enlarge

  12. pNB788

    Plasmid
    #60165
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60165-plasmid-map-sequence-id-96374

    Enlarge

  13. Halo-ATG16L1 HRD

    Plasmid
    #207537
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells
    Depositor
    Jens Schmidt
    Publication
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    207537-plasmid-map-sequence-id-470412

    Enlarge

  14. Halo-TCAB1 HRD

    Plasmid
    #207535
    Purpose
    Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.
    Depositor
    Jens Schmidt
    Publication
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207535-plasmid-map-sequence-id-430972

    Enlarge

  15. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Expression
    Mammalian
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

    Enlarge

  16. pFBD-BirA_CDC20:161-P477

    Plasmid
    #210888
    Purpose
    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    CDC20:S161-P477 (CDC20 Human)
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210888-plasmid-map-sequence-id-424502

    Enlarge

Showing: 1901 - 1920 of 2400 results