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We narrowed to 2,672 results for: pCas

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Showing: 1901 - 1920 of 2672 results

  2. pX330-sgRNA_Dicer_3

    Plasmid
    #68809
    Purpose
    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.
    Depositor
    Constance Ciaudo
    Publication
    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA mouse Dicer1
    Use
    CRISPR and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    68809-plasmid-map-sequence-id-120931

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  3. pCK669

    Plasmid
    #192638
    Purpose
    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Publication
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Sp.pCas9-dxCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dxCas9-NG h…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192638-plasmid-map-sequence-id-381516

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  4. pCK668

    Plasmid
    #192637
    Purpose
    Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Publication
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Sp.pCas9-dCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dCas9-NG ha…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192637-plasmid-map-sequence-id-381515

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  5. pCMV-ABE8.8m-VRQR-P2A-EGFP (BKS971)

    Plasmid
    #242653
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with nVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8.8m-SpCas9-VRQR-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, VRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242653-plasmid-map-sequence-id-488991

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  6. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  9. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Publication
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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  10. RUBY-ABE#2

    Plasmid
    #232176
    Purpose
    T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.
    Depositor
    Yiping Qi
    Publication
    Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265
    Insert
    2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold
    Use
    CRISPR
    Tags
    3xflag and NLS
    Expression
    Plant
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    232176-plasmid-map-sequence-id-459941

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  11. AAVS1_Cas9-hGem_gRNA2

    Plasmid
    #217659
    Purpose
    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA2 targeting the human AAVS1 safe harbour locus
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217659-plasmid-map-sequence-id-438226

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  14. pYPQAT58

    Plasmid
    #223430
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223430-plasmid-map-sequence-id-454181

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  15. pYPQAT27

    Plasmid
    #223399
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223399-plasmid-map-sequence-id-452308

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  17. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161817-plasmid-map-sequence-id-320651

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  18. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid
    #217798
    Purpose
    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR and Lentiviral
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    EF1a
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217798-plasmid-map-sequence-id-432865

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  19. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid
    #217662
    Purpose
    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217662-plasmid-map-sequence-id-440854

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  20. pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965)

    Plasmid
    #242652
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-ABE8.8m-SpG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA, SpG mutations in SpCas9…
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242652-plasmid-map-sequence-id-488985

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Showing: 1901 - 1920 of 2672 results