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We narrowed to 2,008 results for: rigi

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Showing: 1901 - 1920 of 2008 results

  1. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid
    #99231
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    CaMKII
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99231-plasmid-map-sequence-id-219823

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  2. pCMV-dCas13-inactive M3M14nes

    Plasmid
    #157855
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157855-plasmid-map-sequence-id-307628

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  3. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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  6. pETDuet-TFIIE

    Plasmid
    #171083
    Purpose
    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171083-plasmid-map-sequence-id-335640

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  7. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

    Plasmid
    #84483
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available Since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84483-plasmid-map-sequence-id-177044

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  11. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid
    #108273
    Purpose
    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α promoter (1.1kb short version)
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108273-plasmid-map-sequence-id-213551

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  13. pMIG-AVITEV- hNFATc1/aA

    Plasmid
    #74057
    Purpose
    retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc1, isoform alpha-A (NFATC1 Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    mutation G1157A [R235Q] and the silent mutation C…
    Promoter
    pMSCV-LTRs
    Available Since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    74057-plasmid-map-sequence-id-142263

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  14. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

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  16. pLY9

    Plasmid
    #130907
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-2G6, and Ptet
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    130907-plasmid-map-sequence-id-258306

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  17. pLY59

    Plasmid
    #130927
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    Use
    Synthetic Biology
    Tags
    ASV tag and MCP (MS2 coat protein)
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-2G6, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130927-plasmid-map-sequence-id-257328

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  18. pLY57

    Plasmid
    #130926
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130926-plasmid-map-sequence-id-257327

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  19. pLY56

    Plasmid
    #130925
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130925-plasmid-map-sequence-id-257326

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  20. pLY55

    Plasmid
    #130924
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130924-plasmid-map-sequence-id-257309

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Showing: 1901 - 1920 of 2008 results