165,545 results

1. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV1)

   Viral Prep

   #26975-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII

   Tags

   mCherry

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pT3-EF1A-MYC-IRES-lucOS

   Plasmid

   #129776

   Purpose

   Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339

   Depositor

   Amaia Lujambio

   Article

   Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

   Insert

   human MYC (MYC Human)
   

   Use

   Tags

   OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Occludin-N-10

   Plasmid

   #55112

   Purpose

   Localization: Tight Junctions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Occludin (OCLN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK1043

   Plasmid

   #8835

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   tobacco etch virus protease, catalytic domain
   

   Use

   Tags

   MBP and polyarginine

   Expression

   Bacterial

   Mutation

   Autolysis-resistant S219V mutant

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-6xHis-MBP-TEV site-TadA8.20

   Plasmid

   #194702

   Purpose

   Expresses N-terminal MBP fused TadA8.20 in bacteria

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   TadA8.20
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-ShadowG

   Plasmid

   #104620

   Purpose

   Expresses Shadow G in mammalian cell

   Depositor

   Hideji Murakoshi

   Article

   Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

   Insert

   ShadowG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCDH-CMV-human MYCN-EF1-mRFP

   Plasmid

   #193708

   Purpose

   Lentiviral expression of human MYCN

   Depositor

   Martine Roussel

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. dRED

   Plasmid

   #139836

   Purpose

   Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RNase H1 protein contains D210N mutation. dCas9 …

   Promoter

   tet-inducible CMV

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

    Plasmid

    #182925

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Use

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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