We narrowed to 13,463 results for: Ott

1. Lenti_CRISPRi_sgNeg1

   Plasmid

   #166998

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-APEX2-P2A-mKate2

   Plasmid

   #67671

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-P2A-mKate2
   

   Use

   Gateway multisite 3' entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRvACT-mCherry

   Plasmid

   #205002

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSE-DEST_R1R4

   Plasmid

   #97186

   Purpose

   For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector

   Depositor

   Kenneth Scott

   Article

   Lu et al Cancer Res. 2017 May 16. pii: canres.274

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-ReaChR-citrine

   Plasmid

   #169045

   Purpose

   Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   ReaChR-citrine
   

   Use

   Gateway entry cloning vector

   Tags

   citrine

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQUAST-shibire (ts1)

   Plasmid

   #24362

   Depositor

   Liqun Luo

   Article

   Potter et al Volume 141, Issue 3, 536-548, 30 April 2

   Insert

   Shibire (ts1) (shi Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a+GFP-CAMSAP2 CKK

   Plasmid

   #59032

   Purpose

   expresses CKK domain of CAMSAP2 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP2 CKK domain (CAMSAP2 Human)
   

   Use

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pRS416-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158148

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first position of th…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-mKate-P2A-APEX2-NS

   Plasmid

   #67670

   Purpose

   Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-NS
   

   Use

   Gateway multisite middle entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a+GFP-CAMSAP1 CKK

    Plasmid

    #59031

    Purpose

    expresses CKK domain of CAMSAP1 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP1 CKK domain (CAMSAP1 Human)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pFasBac+GFP-CAMSAP3 CC+CKK

    Plasmid

    #59041

    Purpose

    for baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CC+CKK domain (Camsap3 Mouse)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-GBP

    Plasmid

    #67672

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    anti-GFP nanobody
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRF+275Dux4

    Plasmid

    #21293

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX 4 (DUX4 DUX 4, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pMG343

    Plasmid

    #176183

    Purpose

    preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2+ccdB dropout cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUASP dsRed Rab11 WT

    Plasmid

    #44829

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCS2-mqgDUX4

    Plasmid

    #21176

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCW57.1-MBD3L2

    Plasmid

    #106332

    Purpose

    All-in-one lentivector with tet-inducible MBD3L2

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-myc-Sema4A

    Plasmid

    #115171

    Purpose

    Expresses membrane-targeted Sema4A with N-terminal myc tag

    Depositor

    Suzanne Paradis

    Article

    McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

    Insert

    Sema4A (Sema4a Mouse)
    

    Use

    Tags

    membrane targeting sequence and myc

    Expression

    Mammalian

    Mutation

    First 32 amino acids of Sema4A replaced with memb…

    Promoter

    CMV

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUASP dsRed Rab11 CA

    Plasmid

    #44830

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Q70L (constitutive active)

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only