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pOTTC293 - pAAV EF1a V5-synuclein (WT)

Plasmid

#60057

Purpose

An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

alpha-synuclein (SNCA human, Human)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn G51D

Plasmid

#105747

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

Insert

alpha-synuclein G51D (SNCA Human)

Expression

Bacterial

Mutation

G51D

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA-NE

Plasmid

#215486

Purpose

Lentiviral expression of human alpha-synuclein in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-imp α

Plasmid

#119719

Purpose

Expresses mCherry-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importinα (KPNA2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSico_U6-Pan-PGC1a sgRNA

Plasmid

#165426

Purpose

Expression of gRNA against human Total PGC-1a variants

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against human Total PGC-1a variants (PPARGC1A Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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XE130 beta2AR Fz2-Cs2+

Plasmid

#16794

Depositor

Randall Moon

Article

Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

Insert

beta 2 AR Fz2 (Fzd1, Adrb2 Rat)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
mOrange-imp α

Plasmid

#119720

Purpose

Expresses mOrange-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importinα (KPNA2 Human)

Tags

mOrange

Expression

Mammalian

Mutation

contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual wt p110α/p85α

Plasmid

#199302

Purpose

co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

Depositor

Peter Vogt

Article

Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

Insert

His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)

Tags

Avi and His

Expression

Insect

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pM2AH + Slo1

Plasmid

#16195

Depositor

Larry Salkoff

Article

McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

Insert

calcium-activated potassium channel (KCNMA1, Kcnma1 Mouse, Human)

Expression

Mammalian

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pSIN-hSNCA-NE

Plasmid

#102366

Purpose

Lentiviral overexpression of synuclein alpha

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Insert

synuclein alpha (SNCA Human)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

A silent mutation (333bp downstream of ATG) was i…

Promoter

EF-1a

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CSF2RA_CRLF2

Plasmid

#205798

Purpose

Express mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CSF2RA_CRLF2 (CSF2RA, CRLF2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBScMXT + Slo1

Plasmid

#16194

Depositor

Larry Salkoff

Article

McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

Insert

calcium-activated potassium channel (KCNMA1, Kcnma1 Mouse, Human)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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XE129 beta2AR Fz1-pcDNA3.1

Plasmid

#16795

Depositor

Randall Moon

Article

Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

Insert

beta 2 AR Fz1 (Fzd1, Adrb2 Rat)

Use

Xenopus expression

Mutation

Chimera of beta 2 adrenergic receptor (extracellu…

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

Plasmid

#223226

Purpose

guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Nr1d1 (Nr1d1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2R)-NE

Plasmid

#215487

Purpose

Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Mutation

D (second aa) mutated to R

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2E)-NE

Plasmid

#215488

Purpose

Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Mutation

D (second aa) mutated to E

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2R)-GFP

Plasmid

#215490

Purpose

Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

D (second aa) mutated to R

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2E)-GFP

Plasmid

#215491

Purpose

Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

D (second aa) mutated to E

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1

Plasmid

#194172

Purpose

Expresses lncRNA HIF1a-AS1 (spliced)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon2

Plasmid

#194173

Purpose

Expresses lncRNA HIF1a-AS1 Exon1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Mutation

Exon2 was deleted

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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