We narrowed to 5,338 results for: cycs

1. pJ3 SHP2 WT

   Plasmid

   #8317

   Depositor

   Ben Neel

   Article

   Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. DsRed-p150 217–548

   Plasmid

   #51146

   Purpose

   Inhibits dynein-dynactin interaction in mammalian cells

   Depositor

   Trina Schroer

   Article

   Quintyne et al J Cell Biol. 2002 Oct 28;159(2):245-54.

   Insert

   DCTN1 (DCTN1 Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q118R, E340K

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pSCMV-H2B-FT-Slow

   Plasmid

   #157671

   Purpose

   Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

   Depositor

   Shangqin Guo

   Article

   Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

   Insert

   Histone H2B
   

   Use

   Retroviral

   Tags

   Slow-FT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCMV-H2B-FT-Fast

   Plasmid

   #157669

   Purpose

   Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

   Depositor

   Shangqin Guo

   Article

   Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

   Insert

   Histone H2B
   

   Use

   Retroviral

   Tags

   Fast-FT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-E2F-1 wt-pRcCMV

   Plasmid

   #21667

   Depositor

   William Kaelin

   Article

   Krek et al Cell. 1994 Jul 15. 78(1):161-72.

   Insert

   E2F-1 (E2F1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type-

   Promoter

   Available since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-CLCa

   Plasmid

   #196912

   Purpose

   Visualization of clathrin-coated pit formation in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

   Insert

   Clathrin-Coated light chain
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-c/o NL4-3 Env delta CT

   Plasmid

   #139285

   Purpose

   HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   codon optimized HIV-1 Env delta cytoplasmic tail
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of cytoplasmic tail after residue 710

   Promoter

   CMV IE

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-CA-Src-GFP

   Plasmid

   #83469

   Purpose

   Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Src (SRC Chicken)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y527F (constitutively active)

   Promoter

   doxycycline-inducible promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-mCherry-Rab11

   Plasmid

   #174461

   Purpose

   Express mCherry with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with recycling endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFX-EGFP-Rab11

    Plasmid

    #174460

    Purpose

    Express iRFP with recycling endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with recycling endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab11)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAOJ15

    Plasmid

    #91567

    Purpose

    Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tetracycline promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC-aZEA

    Plasmid

    #53286

    Purpose

    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWPI-E2F3

    Plasmid

    #114298

    Purpose

    Constitutive expression of the E2F3 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTU424

    Plasmid

    #178983

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-TO-Vpr-mRuby3-IN

    Plasmid

    #167584

    Purpose

    For producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    Vpr-mRuby3-IN
    

    Use

    Tags

    Vpr-mRuby3

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-Skp2

    Plasmid

    #19946

    Depositor

    Yue Xiong

    Article

    Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

    Insert

    S-phase kinase-associated protein 2 (p45) (SKP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSRalpha-MSV-mouse Cdk4wt-Tk-neo

    Plasmid

    #115525

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

    Insert

    Mouse Cdk4 (Cdk4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

18. EF.deltaBHES1.Ubc.GFP

    Plasmid

    #24982

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    HES1 (HES1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    DNA-binding domain disrupted by E43A, K44A, R47A …

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pTetO-hOSK-CASH-1

    Plasmid

    #188977

    Purpose

    A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

    Depositor

    Sangdun Choi

    Article

    Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

    Insert

    Doxycycline inducible reprogramming donor cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBtet-2xMARS-nGFP-puro

    Plasmid

    #205239

    Purpose

    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Transposon

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    tight TRE promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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