Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,485 results for: firefly luciferase

Clear keywords
Showing: 1921 - 1940 of 4485 results

  2. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196048-plasmid-map-sequence-id-385883

    Enlarge

  5. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168123-plasmid-map-sequence-id-332865

    Enlarge

  7. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Publication
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    COL2A1 regulatory elements
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    97210-plasmid-map-sequence-id-182599

    Enlarge

  8. pG4-CMV-luc

    Plasmid
    #46322
    Depositor
    Steven Brown
    Publication
    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xGal4
    Use
    Luciferase
    Tags
    CMV promoter and luciferase
    Expression
    Mammalian
    Mutation
    five copies of a multimerized GAL4 (5×GAL4) site …
    Promoter
    CMV
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140982-plasmid-map-sequence-id-300744

    Enlarge

  12. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168122-plasmid-map-sequence-id-332862

    Enlarge

  13. pGL4-AARE-luc2P-Hygro

    Plasmid
    #101787
    Purpose
    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)
    Depositor
    Seiichi Oyadomari
    Publication
    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xAARE
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal TATA-box promoter with low basal activity
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101787-plasmid-map-sequence-id-194135

    Enlarge

  17. Lenti-LucS

    Plasmid
    #22778
    Depositor
    Tyler Jacks
    Insert
    SIY
    Use
    Cre/Lox and Lentiviral
    Tags
    luciferase
    Expression
    Mammalian
    Available since
    Dec. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22778-plasmid-map-sequence-id-10426

    Enlarge

Showing: 1921 - 1940 of 4485 results