We narrowed to 152,726 results for: Des

1. pLenti4/V5-DEST-P85(G376R)

   Plasmid

   #40220

   Depositor

   Lynda Chin

   Insert

   P85(G376R) (PIK3R1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Glycine 376 to Arginine

   Promoter

   CMV

   Available since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pET-DEST42-SARA-V5-His6

   Plasmid

   #67431

   Purpose

   Expression of hSara with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDEST40-2XHA-Src-S32-KD

   Plasmid

   #140310

   Purpose

   Expresses HA-tagged human Src-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Contains only the SH3, SH2, and KD regions

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPB-DEST-R1R2-pNeo

   Plasmid

   #141057

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Neomycin resistance gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1/nV5-DEST-Apr1

   Plasmid

   #20135

   Depositor

   Valeri Vasioukhin

   Article

   Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

   Insert

   pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pDEST myc MOV10 K530A

   Plasmid

   #51719

   Purpose

   plasmid expresses myc-tagged MOV10 K530A

   Depositor

   Markus Landthaler

   Article

   Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

   Insert

   MOV10 K530A (MOV10 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K530A

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 5xtetO (tet3) Gene Desert

   Plasmid

   #131339

   Purpose

   gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   Desert-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KN302: pMVP/SB/eCFP-DEST

   Plasmid

   #121863

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KK901: pMVP/SB/Zeo-DEST

   Plasmid

   #121868

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDest-665 (attR4-attR2)

    Plasmid

    #161885

    Purpose

    Multisite Gateway (attR4-attR2) vector for lentivirus production

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti4/V5-DEST-P85(W583del)

    Plasmid

    #40229

    Depositor

    Lynda Chin

    Insert

    P85(W583del) (PIK3R1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acid 583

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCWX-R4DESTR2-PGK-PURO

    Plasmid

    #114319

    Purpose

    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Puromycin resistance

    Depositor

    Patrick Salmon

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST myc MOV10 D645N

    Plasmid

    #51720

    Purpose

    plasmid expresses myc-tagged MOV10 D645N

    Depositor

    Markus Landthaler

    Article

    Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

    Insert

    MOV10 D645N (MOV10 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D645N

    Promoter

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18)

    Plasmid

    #34865

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi4348 targeting
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-DEST42-SAR1-V5-His6

    Plasmid

    #67430

    Purpose

    Expression of hSar1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. 369 pTol-lmo2:egfpDest

    Plasmid

    #73488

    Purpose

    Tol2 vector with lmo2 promoter upstream of EGFP, attR1/R2 cassette and late SV40 pA signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    lmo2 promoter:EGFP-attR1-attR2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti6/V5-DEST-p110 CUX1

    Plasmid

    #100816

    Purpose

    Gateway lentiviral vector expressing p100 CUX1 (amino acids 747-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

    Depositor

    Alain Nepveu

    Article

    Vadnais et al BMC Genomics. 2013 Apr 16;14:258. doi: 1

    Insert

    Hs CUX1 amino acids 747-1505 of P39880 (CUX1 Human)
    

    Use

    Lentiviral

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    contains amino acids 747-1505

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST40-2XHA-Src-S4-UD-S32

    Plasmid

    #140302

    Purpose

    Expresses HA-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the KD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST40-2XHA-Src-S4-S32-KD

    Plasmid

    #140298

    Purpose

    Expresses HA-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Dest53-SHP-1 S591D

    Plasmid

    #21803

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Aspartic acid

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only