We narrowed to 5,083 results for: mcs

1. pcDNA3 PARL-FLAG-CT S70D

   Plasmid

   #13623

   Depositor

   Luca Pellegrini

   Article

   Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

   Insert

   Presenilin-associated rhomboid-like (PARL, PARL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Ser 70 to Asp

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCDH-CMV4

   Plasmid

   #72284

   Purpose

   Express gene of interest under CMV promoter. WPRE has been removed to increase the cloning capacity.

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 25, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCDH-EF1-FLPe

   Plasmid

   #72262

   Purpose

   Express FLPe under EF-1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   FLPe
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. control (pGS701)

   Plasmid

   #160037

   Purpose

   pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRLuc-IVT

   Plasmid

   #205148

   Purpose

   IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA

   Depositor

   Igor Ulitsky

   Insert

   Renilla Luciferase
   

   Use

   Luciferase; In-vitro transcription

   Promoter

   T7

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-nbt300 2n-KalNFB-syt2a

   Plasmid

   #196901

   Purpose

   zebrafish expression

   Depositor

   Reinhard Köster

   Insert

   nbt300 2n-KalNFB-syt2a
   

   Use

   Zebrafish expression

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAF0036 Ef1a-Si74-2a-EGFP

   Plasmid

   #193463

   Purpose

   Expression of Si74 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAF0033 Ef1a-Pa01-2a-EGFP

   Plasmid

   #193460

   Purpose

   Expression of Pa01 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF0035 Ef1a-Nm60-2a-EGFP

   Plasmid

   #193464

   Purpose

   Expression of Nm60 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAF0084 Ef1a-Cp36-2a-EGFP

    Plasmid

    #193462

    Purpose

    Expression of Cp36 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-EGFP-RhoA-T19N

    Plasmid

    #12967

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA DN (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T19N Dominant negative

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-EGFP-Cdc42-T17N

    Plasmid

    #12976

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 DN (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXJ-His-CD276

    Plasmid

    #232662

    Purpose

    transient expresses CD276 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CD276 (CD276 Human)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF0432 Ef1a-Dn29-2a-EGFP

    Plasmid

    #193459

    Purpose

    Expression of Dn29 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Dn29
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. ND6-DdCBE-left side TALE

    Plasmid

    #157842

    Purpose

    expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

    Plasmid

    #199580

    Purpose

    Expresses GCaMP8f in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    His-DIO GCaMP8f
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    hsyn

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 HA E2F1

    Plasmid

    #236431

    Purpose

    transient overexpression of E2F1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F1 (E2F1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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