We narrowed to 10,167 results for: otos

1. RhoB gRNA #1

   Plasmid

   #198721

   Purpose

   Cas9-mediated knockout of RhoB in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoB (Rhob Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RhoB gRNA #2

   Plasmid

   #198722

   Purpose

   Cas9-mediated knockout of RhoB in mammalian cells #2

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoB (Rhob Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RhoB gRNA #3

   Plasmid

   #198723

   Purpose

   Cas9-mediated knockout of RhoB in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoB (Rhob Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RhoC gRNA #1

   Plasmid

   #198724

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RhoC gRNA #2

   Plasmid

   #198725

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #2

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RhoC gRNA #3

   Plasmid

   #198726

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PZac2.1 gfaABC1D-HA-BioID2-SAPAP3

   Plasmid

   #176858

   Purpose

   Expresses SAPAP3 protein fused with BioID2 at the N-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   HA-BioID2-SAPAP3
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH3453

   Plasmid

   #179577

   Purpose

   Pglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   BFP

   Expression

   Worm

   Mutation

   Promoter

   Plgc-55B

   Available Since

   March 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJH2890

   Plasmid

   #179561

   Purpose

   Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Plgc-55B

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUCHR-CD5HA2-P2A-smAID-mClover

    Plasmid

    #110658

    Purpose

    Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CD5HA2-P2A-AID-mClover
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-His-hUbi

    Plasmid

    #31815

    Depositor

    Astar Winoto

    Article

    Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

    Insert

    Ubiquitin (UBB Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRN3P_T3_ABE8e_IVT

    Plasmid

    #201676

    Purpose

    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST-DHFR F[3]-C (LEU2)

    Plasmid

    #177796

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[3]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-HP1alpha

    Plasmid

    #179701

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102886

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCherry-HP1alpha

    Plasmid

    #179703

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-PIF3-mEGFP

    Plasmid

    #100283

    Purpose

    Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N-terminus (1-100 aa) of PIF3 is used

    Promoter

    CAG promoter

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid

    #194328

    Purpose

    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

    Depositor

    Alexander McLellan

    Article

    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Tet-responsive promotor related mutations: G72V i…

    Promoter

    Tight tet-responsive promotor

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge