161,621 results

1. pLenti-CMV-CAG-GFP-EnvA(N2c)

   Plasmid

   #194353

   Purpose

   Lentivirus expressing eGFP and (EnvA-N2c Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies of CVS-N2c strain

   Depositor

   Marco Tripodi

   Article

   Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

   Inserts

   Enhanced Green Fluorescent Protein (eGFP)
   EnvA-CVS-N2c Rabies Glycoprotein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)

   Viral Prep

   #105677-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Promoter

   Syn

   Tags

   FusionRed (Cre-dependent)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

3. DsRed2-C1

   Plasmid

   #54611

   Purpose

   Localization: C1 Cloning Vector, Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   DsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CamKIIa-jGCaMP8m-WPRE (AAV5)

   Viral Prep

   #176751-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CamKIIalpha

   Tags

   None

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

6. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRyl_D17

   Plasmid

   #84611

   Purpose

   Introduces DSB at D17 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18-mini-Tn7T-LAC

   Plasmid

   #64965

   Purpose

   mini-Tn7 vector with Ptac expression cassette

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAAV-DYRK1A-2A-GFP

   Plasmid

   #118270

   Purpose

   Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
   

   Use

   AAV

   Tags

   2A peptide and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV with beta global intron

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQTEV-LRPAP1

    Plasmid

    #31327

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    low density lipoprotein receptor-related protein associated (LRPAP1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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