We narrowed to 24,871 results for: Spr

1. pLenti-LmoCas5

   Plasmid

   #126490

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas5
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-LmoCas6

   Plasmid

   #126491

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

   Plasmid

   #115139

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3.1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti_dCas9-2xAM_hIRF-1

   Plasmid

   #92221

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA targeting human IRF-1 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSC43

   Plasmid

   #104817

   Purpose

   CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoter

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.04g057400
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

   Plasmid

   #115140

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

   Plasmid

   #115138

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS540-pCSDest2-AcrIIC4Hpa

   Plasmid

   #113434

   Purpose

   Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS542-pCSDest2-AcrIIC5Smu

   Plasmid

   #113435

   Purpose

   Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRDA_816

    Plasmid

    #216098

    Purpose

    CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPbuCas13b-gRNA-1

    Plasmid

    #184567

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PlmdCas12e VPR

    Plasmid

    #188516

    Purpose

    Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-VPR

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTW045

    Plasmid

    #185690

    Purpose

    T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

    Insert

    Cas9, Drm1b gRNAs
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTW037

    Plasmid

    #185688

    Purpose

    T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

    Insert

    Cas9, Drm1b gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSC51

    Plasmid

    #104825

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.12g075700, Glyma.11g145900
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV CMV-driven AcrIIA4-2xmiR-1 target sites

    Plasmid

    #120299

    Purpose

    AAV vector for expression of AcrIIA4 with two miR-1 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4-2xmiR-1 binding sites
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

    Plasmid

    #120300

    Purpose

    AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. St1Cas9_LMD-9_sgRNA_pUC19

    Plasmid

    #110627

    Purpose

    U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Insert

    Empty St1Cas9 LMD-9 sgRNA backbone
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

    Plasmid

    #115141

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Orf2mor anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge