We narrowed to 2,586 results for: hells

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pLM1110_LEU2

Plasmid

#168460

Purpose

Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

Depositor

Jef Boeke

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

mRFP1

Use

Synthetic Biology; Yeast assembly vector for big-…

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-hCB2R

Plasmid

#179322

Purpose

Express human cannabinoid 2 receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

Insert

human cannabinoid 2 receptor (CNR2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pED9x (dCas9-KRAB-mCherry)

Plasmid

#163956

Purpose

Lentiviral expression plasmid of sgRNA with mCherry

Depositor

Junwei Shi

Article

Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a_CD WT TET1

Plasmid

#124082

Purpose

Expression of wild-type catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 catalytic domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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hSUMO1 GFP

Plasmid

#118360

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBactin-mNeonGreen-CI

Plasmid

#196850

Purpose

Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 FL

Plasmid

#89835

Purpose

Express GATA3 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBactin-Tubg1-mEGFP

Plasmid

#196870

Purpose

Expression of the gamma-tubulin fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mEGFP (Tubg1 Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA1-gRNA_B

Plasmid

#74375

Purpose

gRNA_B to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA1-gRNA_A

Plasmid

#74374

Purpose

gRNA_A to knockout human AMPK alpha 1 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-roxSTOProx-tRFP-pA-lox

Plasmid

#196888

Purpose

Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

turbo (t)RFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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SNAP-BAF

Plasmid

#227443

Purpose

Mammalian expression of SNAP-BAF

Depositor

David Pellman

Article

Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

Insert

Barrier-to-autointegration factor (BANF1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

Plasmid

#196889

Purpose

Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

lyn-mScarlet

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PHD2

Plasmid

#21401

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN1 (EGLN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pEF1a_FL MUT TET1

Plasmid

#124081

Purpose

Expression of catalytic inactive form of full-length TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - full length, mutated (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

H1672Y, D1674A

Promoter

EF1 alpha

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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hSUMO1-HIS

Plasmid

#118361

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

HIS

Expression

Mammalian

Mutation

Please see Depositor Comments

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD2

Plasmid

#170847

Purpose

AAV backbone for Cre-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a_CD MUT TET1

Plasmid

#124083

Purpose

Expression of mutated catalytic domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - catalytic domain mutated (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

H1672Y, D1674A

Promoter

EF1 alpha

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD1

Plasmid

#170846

Purpose

AAV backbone for transgene expression in cortical interneurons under the control of the Dlx enhancer.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

Plasmid

#153071

Purpose

Lentiviral vector expressing human ZC3H12D mutated (D95N)

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

Mutated ZC3H12D (D95N) (ZC3H12D Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed D95 to N, known to abrogate the RNase enz…

Promoter

EF1α

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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