We narrowed to 2,547 results for: organization Addgene

1. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

   Plasmid

   #127790

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IP-EGFP-LOV2-OMP25

   Plasmid

   #233346

   Purpose

   Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   Omp25 (Synj2bp Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted and N538E

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP12-CB2mutx2-mTagBFP2-HAx2

   Plasmid

   #207147

   Purpose

   Tandem mutant calcium-buffer with rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Calbindin-2 (CALB2 Human)
   

   Tags

   FKBP12, HA, and mTagBFP2

   Expression

   Mammalian

   Mutation

   E40Q, E87Q, E131Q, E175Q, E219Q

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

   Plasmid

   #110854

   Purpose

   Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   sgRNA scaffold with spacer
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   U6

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-Ef1a-SunTag-RPS7-PP7-WPRE

   Plasmid

   #198338

   Purpose

   PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   GCN4x24-RPS7-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops and SunTag

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Cas9-P2A-Puro

   Plasmid

   #110837

   Purpose

   Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-HOT-P2A-Clover-BlastR

   Plasmid

   #138568

   Purpose

   Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   P2A-clover-BlastR
   

   Use

   CRISPR

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TPC2-mCherry

   Plasmid

   #135183

   Purpose

   Expresses TPC2 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC2 (Tpcn2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TPC1-mCherry

   Plasmid

   #135182

   Purpose

   Expresses TPC1 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC1 (Tpcn1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cyto-mTurq2-Apollo-NADP+

    Plasmid

    #193301

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

    Plasmid

    #176395

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-ABERA-P2A-Puro

    Plasmid

    #112675

    Purpose

    Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    ABERA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

    Plasmid

    #196197

    Purpose

    Cas9 + gRNA expression to edit G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TPC2-D276K-G-GECO1.2

    Plasmid

    #207142

    Purpose

    Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Changed Aspartate 276 to Lysine

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry C1 MFF

    Plasmid

    #157760

    Purpose

    Expression of mcherry:MFF in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    MFF (MFF Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry N1 TOMM20

    Plasmid

    #157761

    Purpose

    Expression of TOMM20:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-tomato SOX2-sgRNA

    Plasmid

    #196190

    Purpose

    Editing human SOX2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJM1-TMEM192-FLAG

    Plasmid

    #164977

    Purpose

    Lentiviral vector for TMEM192 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SOX9-T2A-H2B-EGFP repair template

    Plasmid

    #167972

    Purpose

    SOX9 reporter repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 9 (SOX9 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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