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pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantMutationPromoter2x35SAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEN276
Plasmid#69150PurposeIntegration of luciferase operon in Tn7 insertion site of Gram negative bacteriaDepositorInsertluxCDABE
UseLuciferaseTagsExpressionMutationPromoterfrrAvailable sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mNeonGreen2
Plasmid#113727PurposeExpresses the fluorescent protein mNeonGreen2 in a lentiviral backboneDepositorInsertmNeonGreen2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PSEN2_Halo_C_allele
Plasmid#178133PurposeDonor vector for endogenous tagging of human PSEN2 at the C-terminus with halotagInsertHalotag (PSEN2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEN2 F8-2
Plasmid#186033PurposeExpress VHH F8-2 with 3 c-myc tag in E. coli periplasmDepositorInsertVHH F8-2
UseTags6His and MycExpressionBacterialMutationPromoterlacAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEN25-Ub Hs
Plasmid#83232PurposepHis-par2-Ub HsDepositorInsertUbiqutin (UBC Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-FEN2
Plasmid#190616PurposeExpresses a generalist biosensor responsive to bicyclic monoterpenes in E. coliDepositorInsertFEN2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-4
Plasmid#50738PurposeExpresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-4 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ZnGreen2
Plasmid#170781Purposecytosolic ZnGreen2 zinc sensor with 20 uM KdDepositorInsertZnGreen2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-1
Plasmid#50739PurposeExpresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-1 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
psen2_R (OZ578)
Plasmid#35218DepositorInsertZinc finger array targeting psen2 (psen2 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
psen2_L (OZ577)
Plasmid#35217DepositorInsertZinc finger array targeting psen2 (psen2 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCSmEn2ERT(#117)
Plasmid#184079Purposecyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesisDepositorInsert6his-myc-chkEn2-ERT2
UseSynthetic BiologyTags6his-MycExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSmEn2n4ERT(#234)
Plasmid#184082Purposecyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsert6his-myc-chkEn2(n4)-ERT2
UseSynthetic BiologyTags6his-MycExpressionMammalianMutationchkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSm5En2DER4(#1062)
Plasmid#184093Purposecyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsert5myc-chkEn2(C>D)-ERT2
UseSynthetic BiologyTags5xMycExpressionMammalianMutationchkEn2: C175D; ERT2: G400V, M543A, L544A, deleted…PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only
