We narrowed to 48 results for: pKD46

1. BASIC_SEVA_5a7_pKD46.10

   Plasmid

   #178937

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. BASIC_SEVA_27_pKD46.10

   Plasmid

   #178922

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. BASIC_SEVA_17_pKD46.10

   Plasmid

   #178917

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 2, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. BASIC_SEVA_67_pKD46.10

   Plasmid

   #178942

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   March 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. BASIC_SEVA_37_pKD46.10

   Plasmid

   #178927

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   March 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. BASIC_SEVA_47_pKD46.10

   Plasmid

   #178932

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHelper_TS_V2_ampR

   Plasmid

   #214467

   Purpose

   Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Insert

   CspRecT / Bxb-1
   

   Use

   Synthetic Biology

   Promoter

   XylS / AraC

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKDsgRNA-p15

    Plasmid

    #62656

    Purpose

    Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    exo, beta, gam, sgRNA
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJH46

    Plasmid

    #67272

    Purpose

    Conjugally transferable red recombinogenic plasmid

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    traJ, cat, oriT
    

    Use

    Red recombinase

    Promoter

    cat gene promoter from PGNS-BAC

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKDsgRNA-pgaC

    Plasmid

    #154919

    Purpose

    aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pgaC
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKDsgRNA-pheT

    Plasmid

    #154920

    Purpose

    aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pheT
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKDsgRNA-mqo

    Plasmid

    #154921

    Purpose

    aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting mqo
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDsgRNA-hisF

    Plasmid

    #154922

    Purpose

    aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting hisF
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKDsgRNA-ampG

    Plasmid

    #154923

    Purpose

    aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting ampG
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Expression

    Bacterial

    Promoter

    rhamnose

    Available Since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCas

    Plasmid

    #62225

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgR

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Has Service

    Cloning Grade DNA

    Insert

    cas9
    

    Use

    CRISPR; Lambda red recombinase

    Expression

    Bacterial

    Promoter

    native cas9 promoter

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGD248

    Plasmid

    #219498

    Purpose

    Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

    Insert

    Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Expression

    Bacterial

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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