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Viral Prep#51502-AAV9PurposeReady-to-use AAV9 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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AAV pCAG-FLEX-EGFP-WPRE (AAV5)
Viral Prep#51502-AAV5PurposeReady-to-use AAV5 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV1)
Viral Prep#51502-AAV1PurposeReady-to-use AAV1 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV2)
Viral Prep#51502-AAV2PurposeReady-to-use AAV2 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV8)
Viral Prep#51502-AAV8PurposeReady-to-use AAV8 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE (AAV Retrograde)
Viral Prep#51502-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
Plasmid#101215PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1) variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A and R1060APromoterT7Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
Plasmid#101217PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS207 - Bacterial expression plasmid for WT SpCas9
Plasmid#101199PurposeBacterial expression plasmid for WT SpCas9DepositorInsertSpCas9
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ACE2-his expression plasmid
Plasmid#158089PurposeExpresses ACE2 in mammalian cellsDepositorInsertACE2 (ACE2 Human)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
BPK3258 - human expression plasmid for eSpCas9(1.1)
Plasmid#101176PurposeHuman expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationK848A/K1003A/R1060APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK4387 - human expression plasmid for SpCas9 Cluster 5
Plasmid#101191PurposeHuman expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster5(K918A/V922A/R925A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationK918A/V922A/R925APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3645 - human expression plasmid for SpCas9 Cluster 3
Plasmid#101187PurposeHuman expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster3(T657A/G658A/W659A/R661A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationT657A/G658A/W659A/R661APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3770 - human expression plasmid for SpCas9 Cluster 4
Plasmid#101189PurposeHuman expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster4(F491A/M495A/T496A/N497A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationF491A/M495A/T496A/N497APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW2993 - human expression plasmid for SpCas9 Cluster 2
Plasmid#101185PurposeHuman expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationG582A/V583A/E584A/D585A/N588APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)
Plasmid#101178PurposeHuman expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationN692A/M694A/Q695A/H698APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
Plasmid#101209PurposeBacterial expression plasmid for SpCas9-HF1 variantDepositorInsertSpCas9 variant N497A/R661A/Q695A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A, Q695A and Q926APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1
Plasmid#101177PurposeHuman expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationN497A/R661A/Q695A/K848A/Q926A/K1003A/R1060APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain
Plasmid#101205PurposeBacterial expression plasmid for SpCas9 REC3 domainDepositorInsertSpCas9 variant K506–Q712
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK506–Q712PromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant
Plasmid#101202PurposeBacterial expression plasmid for SpCas9∆REC3 variantDepositorInsertSpCas9 variant M1–N497,GGS,V713–D1368
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationM1–N497, GGS, V713–D1368PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only