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Viral Prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)
Viral Prep#52473-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-synP-FLEX-splitTVA-EGFP-B19G (#52473). In addition to the viral particles, you will also receive purified pAAV-synP-FLEX-splitTVA-EGFP-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP
Plasmid#69908PurposeExpresses a GFP circular RNA in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-splitTVA-EGFP
Plasmid#52474PurposeExpresses splitTVA-EGFPDepositorInsertsplitTVA-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterSynapsin-1Available sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-WT Split GFP
Plasmid#69891PurposeExpresses a GFP circular RNA in DrosophilaDepositorInsertGFP, laccase2 (stw Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-splitTVA-EGFP
Plasmid#59332PurposeExpresses splitTVA-P2A-EGFPDepositorInsertsplitTVA950-P2A-EGFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-splitTVA-EGFP-B19G
Plasmid#52473PurposeExpresses splitTVA-EGFP-B19GDepositorHas ServiceAAV1InsertTVA-P2A-EGFP-P2A-B19G
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterSynapsin-1Available sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-splitTVA-EGFP-tTA
Plasmid#100798Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19GDepositorHas ServiceAAV1InsertTVA, EGFP, tet transactivator
UseAAVTagsExpressionMammalianMutationPromotersynapsinAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2
Plasmid#183352PurposeFlp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)DepositorInsertTVA, EGFP and tTA
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES
Plasmid#69909PurposeExpresses a GFP circular RNA containing the sense EMCV IRES in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES
Plasmid#69910PurposeExpresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)
Plasmid#190112PurposeAAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFPDepositorInsertsMMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
UseAAVTagsbpNLS and bpNLS-P2A-eGFPExpressionMutationmutations from RT in PE2 and truncation of RNAse …PromoterEFS and U6, H1Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)
Plasmid#178986PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)
Plasmid#178994PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)
Plasmid#178992PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)
Plasmid#178990PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTET GFP10-FRB/FKBP-GFP11
Plasmid#182497PurposeTripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coilsDepositorInsertsUseTagssplit GFP10 tripartite and split GFP11 tripartiteExpressionBacterialMutationPromoterTETAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-IntCCCre-T2A-CopGFP
Plasmid#160501PurposeExpresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsCopGFPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hSyn-IntCCCre-T2A-CopGFP
Plasmid#160503PurposeExpresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsCopGFPExpressionMammalianMutationPromoterhSynAvailable sinceMarch 12, 2022AvailabilityAcademic Institutions and Nonprofits only