We narrowed to 257 results for: tet off

1. pFGL1252_TetOFF(Hyg)

   Plasmid

   #118992

   Purpose

   TetOFF (promoter-less) cassette with Hygromycin selection

   Depositor

   Naweed Naqvi

   Article

   Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

   Inserts

   tTA+Tnos
   Hygromycin cassette
   Ttub+PtetO
   

   Use

   Fungal expression (in magnaporthe oryzae)

   Tags

   Expression

   Mutation

   Codon optimized for Magnaporthe oryzae

   Promoter

   Aspergillus nidulans TrpC promoter, Ustilago mayd…

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2-SynTetOff-palGFP

   Plasmid

   #190232

   Purpose

   AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   palmitoylation signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTetOFF SNCA-A53T

   Plasmid

   #215372

   Purpose

   All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ala53Thr

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2-SynTetOff-GFP

   Plasmid

   #190231

   Purpose

   AAV vector, high-level expression of EGFP in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2-SynTetOff-myrGFP

   Plasmid

   #190233

   Purpose

   AAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   myristoylation signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1A-SynTetOff-GFP

   Plasmid

   #190230

   Purpose

   Gateway entry vector, high-level expression of EGFP in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-SynTetOff-FGL

   Plasmid

   #190234

   Purpose

   AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   LDLR C-terminal sequence and myristoylation signa…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-tetoff-mVenus-PICdc42

   Plasmid

   #91876

   Purpose

   PI(WT): Photo inhibitable (blue light sensitive)

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Vav2 (VAV2 Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1-MCU-Flag-TetOFF

   Plasmid

   #185657

   Purpose

   Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

    Plasmid

    #229828

    Purpose

    A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Tetoffbidir -Alb-luc

    Plasmid

    #164078

    Purpose

    AAV vector with bidirectional promoter controls expression firefly Luciferase with TRE and Tet-off transactivator

    Depositor

    Shu Uin Gan

    Article

    Gan et al J Gene Med. 2019 Jan;21(1):e3067. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Albumin

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-off TauRD (P301L/V337M)

    Plasmid

    #188572

    Purpose

    Dox-repressible expression of the Tau repeat domain

    Depositor

    Franz-Ulrich Hartl

    Article

    Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

    Insert

    Tau repeat domain (MAPT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P301L and V337M

    Promoter

    pTight TRE

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB-TRE-CRISPRoff-EF1A-TetOn

    Plasmid

    #203355

    Purpose

    Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

    Depositor

    Jonathan Brunger

    Article

    Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

    Insert

    CRISPRoff-TetOn
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-TIGHT-CYCLINF-Tet-OFF

    Plasmid

    #32977

    Depositor

    Michele Pagano

    Insert

    CYCLIN F (CCNF Human)
    

    Use

    Lentiviral

    Tags

    Flag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    P-TIGHT

    Available Since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

15. BS-TRP1-TetOff(7repeats)-GFP-Pho8

    Plasmid

    #207011

    Purpose

    Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid

    #190236

    Purpose

    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NAT-TET-OFF -MET - 3xHA

    Plasmid

    #182526

    Purpose

    Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    7 Copies of TetO Box
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid

    #182527

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    7 Copies of TetO Box
    

    Use

    Tags

    4XFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1-FRB-MCU-Flag-TetOFF

    Plasmid

    #185659

    Purpose

    Tet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitope

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    Mitochondrial calcium uniporter (MCU Human)
    

    Use

    Lentiviral

    Tags

    FRB and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    pTight TRE

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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