158 results

1. AID* Kit

   Plasmid Kit

   #1000000120

   Purpose

   Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

   Depositor

   Helle Ulrich

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

2. EXPRESSYALI

   Plasmid Kit

   #1000000245

   Purpose

   The EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.

   Depositor

   Irina Borodina

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

3. Yeast GoldenBraid Cloning System and Toolkit

   Plasmid Kit

   #1000000138

   Purpose

   Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression

   Depositor

   Elena Caro Bernat

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

4. Target Accelerator Pan-Cancer Mutant Collection

   Plasmid Kit

   #1000000103

   Purpose

   Panel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning system

   Depositor

   Jesse Boehm

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

5. rBiFC Kit

   Plasmid Kit

   #1000000147

   Depositor

   Christopher Grefen

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

6. CRIMPkit

   Plasmid Kit

   #1000000225

   Purpose

   The CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.

   Depositor

   Robert Bryson-Richardson

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

7. EasyCloneYALI- Integrative Vector Set

   Plasmid Kit

   #1000000140

   Purpose

   Contains integrative plasmids suitable for (over-)expression of (heterologous) genes in Y. lipolytica strains

   Depositor

   Irina Borodina

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

8. Fungal Toolkit for Modular Cloning (FTK)

   Plasmid Kit

   #1000000191

   Purpose

   Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.

   Depositor

   Arnold Driessen

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

9. EasyCloneYALI- CRISPR/Cas9 Set

   Plasmid Kit

   #1000000141

   Purpose

   Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9

   Depositor

   Irina Borodina

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

10. B. subtilis Single Gene Deletion Library--Kanamycin

    Plasmid Kit

    #1000000115

    Purpose

    B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.

    Depositor

    Carol Gross

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

11. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit

    #1000000109

    Purpose

    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.

    Depositor

    Bob Goldstein

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

12. MultiGreen

    Plasmid Kit

    #1000000250

    Purpose

    The MultiGreen kit enables users to assemble GreenGate-compatible multigene plant transformation vectors.

    Depositor

    Wayne Parrott

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pCut plasmid toolkit

    Plasmid Kit

    #1000000118

    Purpose

    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.

    Depositor

    Aindrila Mukhopadhyay

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

14. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit

    #1000000014

    Purpose

    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.

    Depositor

    David Root, William Hahn

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

15. openCIDAR MoClo Toolkit

    Plasmid Kit

    #1000000227

    Purpose

    This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.

    Depositor

    Eric Young

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

16. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit

    #1000000030

    Purpose

    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

    Depositor

    Takashi Yamamoto

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Shen Lab DdCBE assembly kit

    Plasmid Kit

    #1000000212

    Purpose

    The DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.

    Depositor

    Bin Shen

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

18. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit

    #1000000060

    Purpose

    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.

    Depositor

    Ian Frew

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. NT-CRISPR plasmid collection

    Plasmid Kit

    #1000000189

    Purpose

    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.

    Depositor

    Anke Becker

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

20. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit

    #1000000116

    Purpose

    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.

    Depositor

    Carol Gross

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only