155 results
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Plasmid Kit#1000000156PurposeThe MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.DepositorAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only
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RAS Pathway 2.0 Clone Collection
Plasmid Kit#1000000070PurposeCollection contains 180 genes in the âRAS pathway version 2.0â, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning PlatformDepositorAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)
Plasmid Kit#1000000221PurposeA positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.DepositorAvailable sinceJune 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
SapTrap Kit
Plasmid Kit#1000000077PurposeSapTrap: modular toolkit for building CRISPR/Cas9 targeting vectors to insert genetic tags in C. elegans genome. 21 vector assembly plasmids, Unc-32::GFP targeting vector, 5 Cre/Flp expression vectorsDepositorAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fire Lab C. Elegans Kit
Plasmid Kit#1000000001PurposeWorm (C. elegans) vector set comprised of lacZ/GFP fusions, exon and intron GFP tagging vectors, RNAi controls, and hairpin sequences for testing the ability of promoters to in vivo trigger RNAiDepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
FX cloning Kit
Plasmid Kit#1000000039PurposeInsert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systemsDepositorAvailable sinceApril 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Komagataeibacter Tool Kit (KTK)
Plasmid Kit#1000000216PurposeThe Komagataeibacter Tool Kit (KTK) is a standardized modular cloning system based on Golden Gate DNA assembly.DepositorAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
RAS mutant clone collection
Plasmid Kit#1000000089PurposeWild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clonesDepositorAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9
Plasmid Kit#1000000154PurposeVectors for integration using short homology and CRISPR/Cas9 to produce knockout alleles with and without a fluorescent reporter, fusion proteins with endogenous genes, and Cre drivers.DepositorAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Alexandrov Cell-Free Protein Expression Kit (PEK)
Plasmid Kit#1000000065PurposeSet of 89 ORFs cloned into the pCellFree vector to enable protein expression in any in vitro translation system; can perform statistically relevant benchmarking of cell-free expression systemsDepositorAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Yeast GoldenBraid Cloning System and Toolkit
Plasmid Kit#1000000138PurposeModular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expressionDepositorAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE Dicot Genome Editing Kit
Plasmid Kit#1000000084PurposeGolden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of âgenome editing recipientâ and âsgRNA shuttleâ vectors.DepositorAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
C. elegans miniMos transgenesis kit
Plasmid Kit#1000000031Purpose29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.DepositorAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
MXS Chaining Kit
Plasmid Kit#1000000064PurposeMXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signalsDepositorAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Yeast GPCR-sensor Toolkit
Plasmid Kit#1000000157PurposeThe Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.DepositorAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmTRQ2/mVenus FLIM/FRET Kit
Plasmid Kit#1000000149DepositorAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Diatom uLoop Assembly Kit
Plasmid Kit#1000000238PurposeThe collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.DepositorAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Joung Lab REAL Assembly TALEN Kit
Plasmid Kit#1000000017PurposeUsed to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.DepositorAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
Rinehart & Söll Phosphoprotein Synthesis Kit
Plasmid Kit#1000000020PurposeAllows Sep incorporation (directed by UAG) into a protein by an E. coli strain that harbors a Sep-accepting tRNA (tRNASep), its cognate SepâtRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep).DepositorAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
Vaccinia Virus ORF Library
Plasmid Kit#1000000009PurposeVaccinia virus (VACV) expression library containing each of the predicted open reading frames based on an unpublished sequence of vaccinia virus strain WRDepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Lung Cancer Mutant Collection
Plasmid Kit#1000000104PurposePanel of lung adenocarcinoma alleles that have undergone basic, functional screening; full-length human clones (180 mutant and 45 corresponding wild-type) and 82 wild-type experssion controlsDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myristoylated Kinase Library
Plasmid Kit#1000000012Purpose190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Plasmid Kit#1000000005PurposeCan be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGCS vector kit
Plasmid Kit#1000000107PurposeA series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.DepositorAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium Expression Vector Kit v1.0
Plasmid Kit#1000000010PurposeContains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
MultiSite Gateway plasmid toolKit
Plasmid Kit#1000000050PurposeCloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modulesDepositorAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Genetic Pathway Reference Set
Plasmid Kit#1000000105PurposeCollection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processesDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
ScEnSor Kit
Plasmid Kit#1000000215PurposeThis three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.DepositorAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hamdoun lab FP fusion plasmids
Plasmid Kit#1000000033PurposeScreen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)DepositorAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Start-Stop Assembly Toolkit
Plasmid Kit#1000000152PurposeStart-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.DepositorAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only